|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1985-9-4
|
|
pubmed:abstractText |
We have investigated the early events of pre-mRNA splicing in vitro by sucrose gradient sedimentation analysis. Time course experiments revealed the assembly, in two steps, of a large (50S) pre-mRNA splicing complex, preceded by formation of two other complexes that sediment at approximately 22S and 35S. Pre-mRNA and the intermediates and products of the in vitro splicing reaction cosediment with the 50S complex, while only pre-mRNA is associated with the 22S and 35S complexes. No splicing is observed in the absence of a 50S complex. Formation of the 50S complex requires ATP, whereas formation of the 22S and 35S complexes does not. U-snRNPs are necessary for assembly of the 35S and the 50S complexes but not for assembly of the 22S complex. Analysis with mutant substrate RNAs demonstrated that a polypyrimidine stretch near the 3' splice site and an intact 5' splice site are absolutely required for splicing complex formation.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
42
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
355-67
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:3160483-Adenosine Triphosphate,
pubmed-meshheading:3160483-Adenoviruses, Human,
pubmed-meshheading:3160483-Base Sequence,
pubmed-meshheading:3160483-Centrifugation, Density Gradient,
pubmed-meshheading:3160483-Kinetics,
pubmed-meshheading:3160483-Mutation,
pubmed-meshheading:3160483-Nucleic Acid Precursors,
pubmed-meshheading:3160483-RNA, Messenger,
pubmed-meshheading:3160483-RNA, Viral,
pubmed-meshheading:3160483-RNA Precursors,
pubmed-meshheading:3160483-RNA Splicing,
pubmed-meshheading:3160483-Ribonucleoproteins,
pubmed-meshheading:3160483-Ribonucleoproteins, Small Nuclear
|
|
pubmed:year |
1985
|
|
pubmed:articleTitle |
Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
