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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0022688,
umls-concept:C0024264,
umls-concept:C0026336,
umls-concept:C0086418,
umls-concept:C0205248,
umls-concept:C0205296,
umls-concept:C0431085,
umls-concept:C0597032,
umls-concept:C0600432,
umls-concept:C1511938,
umls-concept:C1533691,
umls-concept:C1879547,
umls-concept:C2603343
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pubmed:issue |
6
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pubmed:dateCreated |
1985-7-29
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pubmed:abstractText |
A subpopulation of low density granular lymphocytes that express the natural killer (NK) cell-associated Leu-11 antigen (IgG Fc receptor) were stimulated directly by coculture with an NK-sensitive tumor cell, K562. T lymphocytes (Leu-11-) responded only weakly when cocultured with K562. The response of Leu-11+ cells apparently did not require exogeneous factors or accessory cells. The K562-activated cells retained expression of Leu-11 antigen, acquired activation antigens, and were highly cytotoxic against NK-sensitive and -insensitive tumor cells. Anti-IL-2 receptor monoclonal antibody minimally inhibited the activation of Leu-11+ cells by K562, but completely inhibited the phytohemagglutinin-induced activation of the Leu-11- cells from the same individual. Leu-11+ cells can be divided into Leu-7-11+ and Leu-7+11+ subpopulations using anti-Leu-7 antibody. These subsets were separated by two-color fluorescence-activated cell sorting and cocultured with K562. Proliferation by Leu-7-11+ cells was significantly greater than by Leu-11+7+ cells. Leu-7+11- granular lymphocytes and T lymphocytes (Leu-7-11-) typically proliferated only weakly when cocultured with K562. A proportion of the Leu-7-11+ cells acquired Leu-7 antigen after stimulation with K562, whereas the phenotype of Leu-7+11+, Leu-7+11-, and Leu-7-11- subsets was unaffected. These results demonstrate a developmental relationship between the Leu-7-11+ and Leu-7+11+ lymphocytes and suggest that Leu-7 antigen may be expressed late in the differentiation pathway of NK cells. The direct activation of highly purified Leu-11+ cells by coculture with K562 provides an in vitro model with which to study the activation and maturation of human NK cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-157371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-158433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-308088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-3871216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-4137183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-566386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6092949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6166701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6176642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6180009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6191218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6201541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6203975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6208306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6213707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6220056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6220110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6225799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6231353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6233369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6233371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6355362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6367046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6430709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6434688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6601144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6815536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6833758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6848618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6975321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-6980948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3159818-95205
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1464-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3159818-Antibodies, Monoclonal,
pubmed-meshheading:3159818-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:3159818-Antigens, Surface,
pubmed-meshheading:3159818-Cell Differentiation,
pubmed-meshheading:3159818-Cell Line,
pubmed-meshheading:3159818-Humans,
pubmed-meshheading:3159818-Killer Cells, Natural,
pubmed-meshheading:3159818-Lymphocyte Activation,
pubmed-meshheading:3159818-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:3159818-Neoplasms,
pubmed-meshheading:3159818-T-Lymphocytes
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pubmed:year |
1985
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pubmed:articleTitle |
A model for the differentiation of human natural killer cells. Studies on the in vitro activation of Leu-11+ granular lymphocytes with a natural killer-sensitive tumor cell, K562.
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pubmed:publicationType |
Journal Article,
In Vitro
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