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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1985-6-6
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pubmed:abstractText |
The subcellular distribution of protein kinase C (PK C) was examined in thyrotropin-releasing hormone (TRH)--responsive GH3 pituitary cells. TRH treatment, which is known to stimulate polyphosphoinositide turnover and diacylglycerol generation, resulted in a rapid (less than or equal to 15 sec) and transient redistribution of the enzyme from cytosol to membrane fraction. Other agents which either stimulate PK C directly (1-oleoyl-2-acetyl-sn-glycerol and 12-O-tetradecanoyl phorbol-13-acetate) or elevate cellular diglyceride levels (phospholipase C) also promoted a redistribution of the enzyme from cytosol to membrane. These results provide evidence for the concept that cell-surface receptor-mediated phosphoinositide breakdown activates PK C. It appears that translocation of PK C to the membrane is an early step in the cellular activation of this enzyme.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
531-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3158313-Animals,
pubmed-meshheading:3158313-Barium,
pubmed-meshheading:3158313-Biological Transport,
pubmed-meshheading:3158313-Cell Line,
pubmed-meshheading:3158313-Cell Membrane,
pubmed-meshheading:3158313-Cytosol,
pubmed-meshheading:3158313-Pituitary Neoplasms,
pubmed-meshheading:3158313-Potassium,
pubmed-meshheading:3158313-Protein Kinase C,
pubmed-meshheading:3158313-Protein Kinases,
pubmed-meshheading:3158313-Rats,
pubmed-meshheading:3158313-Tetradecanoylphorbol Acetate,
pubmed-meshheading:3158313-Thyrotropin-Releasing Hormone,
pubmed-meshheading:3158313-Type C Phospholipases
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pubmed:year |
1985
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pubmed:articleTitle |
Protein kinase C translocates from cytosol to membrane upon hormone activation: effects of thyrotropin-releasing hormone in GH3 cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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