Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1985-5-23
|
| pubmed:abstractText |
Previous studies have shown that monoclonal anti-Leu-8 antibody identifies functionally distinct subpopulations within both the Leu-2 (T8+) and Leu-3 (T4+) lineages of human T lymphocytes. We now report in detail on the tissue distribution of the Leu-8 antigen and on extensive functional studies of T cells subsets distinguished by their expression or lack of expression of this marker. Leu-8 is present on a wide variety of hematologic cells, including granulocytes, T and B lymphocytes, monocytes, and null or NK cells. Within lymph nodes and tonsils, Leu-8 is absent from both B and T cells within germinal centers but is present on nearly all paracortical lymphocytes. Leu-8 is present on most but not all EBV-transformed B cell lines, reflecting its presence on a subset of normal peripheral blood B cells. None of six malignant T cell lines tested were Leu-8+, whereas most circulating T cells are Leu-8+. Although standard immunoprecipitation techniques failed to demonstrate any specific bands on SDS polyacrylamide gels, the antigenic determinant recognized by anti-Leu-8 is protein or protein-associated, because brief treatment of target cells with pronase abrogated binding of anti-Leu-8. Both Leu-3+8+ and Leu-3+8- cells proliferated in response to several soluble antigens and to autologous and allogeneic non-T cells. Nonetheless, nearly all of the helper T cells for PWM- and AMLR-induced PFC were contained within the Leu3+8- subset. Optimal suppression of the PWM-induced PFC response required both Leu-2+8+ and Leu-2+8- cells, and irradiation of either subset with 3000 R abrogated the capacity of the recombined subsets to effect suppression. In contrast to help for B cell differentiation, both Leu-3+8+ and Leu-3+8- cells were capable of amplifying the development of allospecific T killer cells; precursor and effector T killer cells could be found within both Leu-2+8+ and Leu-2+8- subpopulations. The correlation between Leu-8 phenotype and selected immune functions of T cells (and B cells; see companion paper) indicates that anti-Leu-8 distinguishes important immunoregulatory T and B lymphocyte subsets in man.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
134
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2995-3002
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:3156924-Animals,
pubmed-meshheading:3156924-Antibodies, Monoclonal,
pubmed-meshheading:3156924-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:3156924-Antigens, Surface,
pubmed-meshheading:3156924-B-Lymphocytes,
pubmed-meshheading:3156924-Binding, Competitive,
pubmed-meshheading:3156924-Cell Differentiation,
pubmed-meshheading:3156924-Cytotoxicity, Immunologic,
pubmed-meshheading:3156924-Granulocytes,
pubmed-meshheading:3156924-Hemolytic Plaque Technique,
pubmed-meshheading:3156924-Humans,
pubmed-meshheading:3156924-Killer Cells, Natural,
pubmed-meshheading:3156924-Lymphocyte Activation,
pubmed-meshheading:3156924-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:3156924-Lymphocytes,
pubmed-meshheading:3156924-Monocytes,
pubmed-meshheading:3156924-Pokeweed Mitogens,
pubmed-meshheading:3156924-Rabbits,
pubmed-meshheading:3156924-T-Lymphocytes
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Functional characterization of human T lymphocyte subsets distinguished by monoclonal anti-leu-8.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|