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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1985-3-14
|
| pubmed:abstractText |
H-2-lacking murine embryonal carcinoma (EC) cells have been proposed as universal targets for natural killer (NK) effectors from different species because their killing appeared to be uncomplicated by potential T cell effector mechanisms (Stern, P. L. et al., Int. J. Cancer 1981. 27:679). While some previous studies had shown that murine cytotoxic T cells were unable to lyse EC cells, rat T killers are shown here to be active against these targets and to be distinguishable from NK cells. Percoll density fractionation of rat peripheral blood lymphocytes enriches in parallel for NK-mediated lysis of both EC or YAC target cells. These NK cells unlike T cells, do not mediate lectin-dependent and cell-mediated cytotoxicity (LDCC) of NK-insensitive target cells. This procedure is thought to reveal the total cytolytic potential of stimulated T cell populations, regardless of specificity. In contrast to previous results with mice, we found that allogeneically primed rat cytotoxic T cells can kill murine EC cells in LDCC and, further, that rat cytotoxic T cells, generated by stimulation with mouse spleen cells in vitro, can lyse murine EC cells directly. This demonstration of T cell lysis of EC cells suggests that either there is a novel mechanism of lysis operating without requirement for major histocompatibility complex (MHC) structures, or EC cells express some hitherto unidentified MHC-like structures on their cell surface.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0014-2980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
59-65
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:3155686-Animals,
pubmed-meshheading:3155686-Cell Line,
pubmed-meshheading:3155686-Cell Separation,
pubmed-meshheading:3155686-Centrifugation, Density Gradient,
pubmed-meshheading:3155686-Cytotoxicity, Immunologic,
pubmed-meshheading:3155686-Embryonal Carcinoma Stem Cells,
pubmed-meshheading:3155686-H-2 Antigens,
pubmed-meshheading:3155686-Killer Cells, Natural,
pubmed-meshheading:3155686-Lectins,
pubmed-meshheading:3155686-Lymphocyte Activation,
pubmed-meshheading:3155686-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:3155686-Mice,
pubmed-meshheading:3155686-Mice, Inbred BALB C,
pubmed-meshheading:3155686-Mice, Inbred C57BL,
pubmed-meshheading:3155686-Mice, Inbred CBA,
pubmed-meshheading:3155686-Neoplastic Stem Cells,
pubmed-meshheading:3155686-Rats,
pubmed-meshheading:3155686-Rats, Inbred F344,
pubmed-meshheading:3155686-T-Lymphocytes,
pubmed-meshheading:3155686-Teratoma
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Rat natural killer cell and cytotoxic T cell lysis of H-2-negative murine embryonal carcinoma cells.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|