Linked Life Data

3155468

Source:http://linkedlifedata.com/resource/pubmed/id/3155468

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-1-22
pubmed:abstractText
We have raised an antibody to the IgG Fc receptor (FcR) of human mononuclear phagocytes by immunizing a goat with FcR purified by ligand affinity from a human monocyte line (U937). This antiserum, which inhibited the binding of IgG ligand to the receptors on U937, precipitated from detergent lysates of surface-radioiodinated U937 cells a 72 Kd sialoglycoprotein (p72) identified as the FcR by several previously published criteria. Two other bands seen in autoradiograms of SDS gels were precipitated by this antiserum: a 40 to 43 Kd band that co-purified with p72 and a 170 Kd protein that was not present in the immunogen. Fractionation of the IgG of this antiserum into two subclasses yielded one subclass (IgG1) in which anti-p72 activity was considerably enriched relative to antibody activities against other molecules. This antiserum precipitated p72 not only from U937 but from HL60 cells and from human peripheral blood monocytes as well, indicating common antigens on the p72 molecules from these three cells. However, p72 was not recovered from lysates of surface-iodinated human polymorphonuclear leukocytes or murine macrophage lines. Anti-p72 activity was not completely removed by adsorption with intact U937, suggesting that the antiserum recognizes portions of p72 that are not exteriorly disposed, perhaps noncarbohydrate portions of the molecule. We expect this antiserum to be useful for a number of studies of receptor structure and function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:volume
134
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-70
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Characterization of a polyvalent antibody directed against the IgG Fc receptor of human mononuclear phagocytes.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.