| pubmed-article:3146413 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3146413 | lifeskim:mentions | umls-concept:C0072523 | lld:lifeskim |
| pubmed-article:3146413 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:3146413 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:3146413 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:3146413 | pubmed:dateCreated | 1989-3-16 | lld:pubmed |
| pubmed-article:3146413 | pubmed:abstractText | We have previously reported that pS2 mRNA expressed in cultured epithelial cells derived from a hormone-dependent breast carcinoma (MCF-7 cells) is also expressed in mucosa cells of normal human stomach. This mRNA encodes a putative 84 amino-acid-long protein, which is secreted by both cell types after elimination of a signal peptide. We report here the purification of the pS2 protein, its trypsin digestion and amino-acid sequencing. The MCF-7 cell-secreted protein is 60 amino-acid-long and its sequence is in complete agreement with that deduced from the mRNA sequence. The presence of an N-terminal glutamic acid indicates that the signal peptidase releases a 24 amino-acid-long signal peptide. Analysis of tryptic peptides derived from the secreted gastric pS2 protein indicates that the signal peptide and the sequence of the first 48 amino-acids are identical to those of secreted MCF-7 pS2 protein, although the N-terminal amino-acid of the gastric protein may be cyclized as a pyroglumatic acid. | lld:pubmed |
| pubmed-article:3146413 | pubmed:language | fre | lld:pubmed |
| pubmed-article:3146413 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3146413 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3146413 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3146413 | pubmed:issn | 0764-4469 | lld:pubmed |
| pubmed-article:3146413 | pubmed:author | pubmed-author:ChambonPP | lld:pubmed |
| pubmed-article:3146413 | pubmed:author | pubmed-author:LepagePP | lld:pubmed |
| pubmed-article:3146413 | pubmed:author | pubmed-author:SinL DLD | lld:pubmed |
| pubmed-article:3146413 | pubmed:author | pubmed-author:RoitschCC | lld:pubmed |
| pubmed-article:3146413 | pubmed:author | pubmed-author:DiemunschPP | lld:pubmed |
| pubmed-article:3146413 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3146413 | pubmed:volume | 307 | lld:pubmed |
| pubmed-article:3146413 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3146413 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3146413 | pubmed:pagination | 825-31 | lld:pubmed |
| pubmed-article:3146413 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:3146413 | pubmed:meshHeading | pubmed-meshheading:3146413-... | lld:pubmed |
| pubmed-article:3146413 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3146413 | pubmed:articleTitle | [Primary structure of human protein pS2]. | lld:pubmed |
| pubmed-article:3146413 | pubmed:affiliation | Laboratoire de Génétique moléculaire des Eucaryotes du C.N.R.S., Strasbourg. | lld:pubmed |
| pubmed-article:3146413 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3146413 | pubmed:publicationType | English Abstract | lld:pubmed |
| entrez-gene:7031 | entrezgene:pubmed | pubmed-article:3146413 | lld:entrezgene |
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