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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1989-3-16
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pubmed:abstractText |
We have previously reported that pS2 mRNA expressed in cultured epithelial cells derived from a hormone-dependent breast carcinoma (MCF-7 cells) is also expressed in mucosa cells of normal human stomach. This mRNA encodes a putative 84 amino-acid-long protein, which is secreted by both cell types after elimination of a signal peptide. We report here the purification of the pS2 protein, its trypsin digestion and amino-acid sequencing. The MCF-7 cell-secreted protein is 60 amino-acid-long and its sequence is in complete agreement with that deduced from the mRNA sequence. The presence of an N-terminal glutamic acid indicates that the signal peptidase releases a 24 amino-acid-long signal peptide. Analysis of tryptic peptides derived from the secreted gastric pS2 protein indicates that the signal peptide and the sequence of the first 48 amino-acids are identical to those of secreted MCF-7 pS2 protein, although the N-terminal amino-acid of the gastric protein may be cyclized as a pyroglumatic acid.
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pubmed:language |
fre
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/TFF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins
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pubmed:status |
MEDLINE
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pubmed:issn |
0764-4469
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
307
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
825-31
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3146413-Alkylation,
pubmed-meshheading:3146413-Amino Acid Sequence,
pubmed-meshheading:3146413-Base Sequence,
pubmed-meshheading:3146413-Breast Neoplasms,
pubmed-meshheading:3146413-Chromatography, High Pressure Liquid,
pubmed-meshheading:3146413-Culture Media,
pubmed-meshheading:3146413-Cysteine,
pubmed-meshheading:3146413-Gastric Mucosa,
pubmed-meshheading:3146413-Humans,
pubmed-meshheading:3146413-Molecular Sequence Data,
pubmed-meshheading:3146413-Neoplasm Proteins,
pubmed-meshheading:3146413-Oxidation-Reduction,
pubmed-meshheading:3146413-Peptide Fragments,
pubmed-meshheading:3146413-Proteins,
pubmed-meshheading:3146413-RNA, Messenger,
pubmed-meshheading:3146413-Trypsin,
pubmed-meshheading:3146413-Tumor Cells, Cultured,
pubmed-meshheading:3146413-Tumor Suppressor Proteins
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pubmed:year |
1988
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pubmed:articleTitle |
[Primary structure of human protein pS2].
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pubmed:affiliation |
Laboratoire de Génétique moléculaire des Eucaryotes du C.N.R.S., Strasbourg.
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pubmed:publicationType |
Journal Article,
English Abstract
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