Linked Life Data

3141419

Source:http://linkedlifedata.com/resource/pubmed/id/3141419

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
33
pubmed:dateCreated
1988-12-20
pubmed:abstractText
Gb (b for botulinum) is an Mr 21,000-22,000 guanine nucleotide binding protein which is specifically ADP-ribosylated by an ADP-ribosyltransferase produced by types C1 and D Clostridium botulinum (Morii, N., Sekine, A., Ohashi, Y., Nakao, K., Imura, H., Fujiwara, M., and Narumiya, S. (1988) J. Biol. Chem. 263, 12420-12426). In this study, we have identified the partial amino acid sequence of this protein. Purified Gb was digested with various proteases, and the proteolytic fragments were separated by high performance liquid chromatography. Isolated peptides were subjected to protein sequencing by an automated pulse-liquid phase protein sequenator. Eight partial amino acid sequences consisting of a total of 105 amino acid residues were obtained from 16 peptides. Computerized homology analysis revealed that they are homologous to the sequence deduced from cDNA of the rho genes. About 88% of the amino acid residues was identical between the sequenced Gb fragments and the corresponding regions of the Aplysia rho gene product. The result strongly suggests that Gb is a putative rho gene product.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17255-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Substrate for botulinum ADP-ribosyltransferase, Gb, has an amino acid sequence homologous to a putative rho gene product.
pubmed:affiliation
Department of Pharmacology, Kyoto University Faculty of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't