Linked Life Data

3140899

Source:http://linkedlifedata.com/resource/pubmed/id/3140899

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-12-20
pubmed:abstractText
The in vivo, interorganelle movement of phospholipids synthesized by different biosynthesis routes has been investigated in rat liver. Rats were injected with [methyl-3H]choline, [1-3H]ethanolamine or [3-3H]serine into the portal vein. Subcellular membranes (endoplasmic reticulum, Golgi apparatus, plasma membrane and mitochondria) were isolated, and the specific radioactivites of the phospholipids in each membrane were determined. There was a very rapid distribution of phospholipids from their sites of synthesis to the other organelles. In the plasma membrane, for example, the specific radioactivity of phosphatidylcholine derived from choline, ethanolamine or serine was as high as, or higher than, in the endoplasmic reticulum at all times examined. In addition, the specific radioactivity of phosphatidylserine (derived from serine) in the plasma membrane was approximately double that in the endoplasmic reticulum, even though the latter is the major site of phosphatidylserine synthesis. There was no evidence for the sequential flow of phospholipid from the endoplasmic reticulum, via the Golgi apparatus, to the plasma membrane. The experiments also demonstrated that the various subcellular membranes were labeled to different extents with phospholipids synthesized from different biosynthetic routes. It is unlikely that there is sufficient phospholipid biosynthetic enzyme activity in subcellular organelles other than the endoplasmic reticulum (Vance, J.E. and Vance, D.E. (1988) J. Biol. Chem. 263, 5898-5909) and the mitochondria for phosphatidylserine decarboxylase, to account for the efficient labeling of phospholipids of the plasma membrane, mitochondria and Golgi apparatus. The data suggest that although phospholipids can move very rapidly from one organelle to another, and within the plane of the lipid bilayer, there is neither a rapid mixing of newly synthesized phospholipids with the endogenous phospholipid pool, nor a rapid mixing of phospholipids derived from different biosynthetic origins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
963
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Compartmentalization and differential labeling of phospholipids of rat liver subcellular membranes.
pubmed:affiliation
Department of Medicine, University of Alberta, Edmonton, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't