Linked Life Data

3140682

Source:http://linkedlifedata.com/resource/pubmed/id/3140682

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-10-31
pubmed:abstractText
Immunoaffinity-purified antibodies against soluble Schistosoma mansoni egg antigens (SEA) were isolated from the sera of patients with schistosomiasis mansoni. Similarly, antibodies against Trypanosoma cruzi epimastigote antigens were obtained from sera of patients with Chagas' disease. These antibody preparations were used in culture to demonstrate the presence of anti-idiotypic T lymphocytes in peripheral blood mononuclear cell preparations from patients with either schistosomiasis mansoni or Chagas' disease, or with both of these infections. Only cells from patients with schistosomiasis or both infections proliferated upon exposure to the anti-SEA antibodies. Conversely, only cells from patients with Chagas' disease or both infections responded to anti-epimastigote antibodies. Western blot analysis of SEA and epimastigote antigens, developed by patients' sera or by immunoaffinity-purified antibody preparations, substantiated that anti-SEA immunoaffinity-purified antibodies only reacted with components of SEA, and anti-epimastigote immunoaffinity-purified antibodies only reacted with components of epimastigote antigenic preparation. These studies demonstrate the presence of anti-idiotypic T lymphocytes in the peripheral blood of patients with schistosomiasis or Chagas' disease which are specific for idiotypes generated during these infections.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0002-9637
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
288-94
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:3140682-Animals, pubmed-meshheading:3140682-Antibodies, Helminth, pubmed-meshheading:3140682-Antibodies, Protozoan, pubmed-meshheading:3140682-Antigen-Antibody Reactions, pubmed-meshheading:3140682-Antigens, Helminth, pubmed-meshheading:3140682-Antigens, Protozoan, pubmed-meshheading:3140682-Blotting, Western, pubmed-meshheading:3140682-Chagas Disease, pubmed-meshheading:3140682-Chromatography, Affinity, pubmed-meshheading:3140682-Humans, pubmed-meshheading:3140682-Immunoglobulin Idiotypes, pubmed-meshheading:3140682-Leukocytes, Mononuclear, pubmed-meshheading:3140682-Lymphocyte Activation, pubmed-meshheading:3140682-Schistosoma mansoni, pubmed-meshheading:3140682-Schistosomiasis mansoni, pubmed-meshheading:3140682-T-Lymphocytes, pubmed-meshheading:3140682-Trypanosoma cruzi
pubmed:year
1988
pubmed:articleTitle
Idiotypic/anti-idiotypic interactions in schistosomiasis and Chagas' disease.
pubmed:affiliation
Departamentos de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Brazil.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't