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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-10-21
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pubmed:abstractText |
A method has been designed to prepare inside-out thylakoid vesicles from a cyanobacterial species (Phormidium laminosum). Everted thylakoid vesicles could be generated by Yeda press treatment after induced membrane pairing. Membrane pairing was induced either by addition of high concentrations of Mg2+ ions or by lowering the pH of the fragmentation media. The inside-out vesicles were isolated by aqueous polymer two-phase partition. The membrane orientation was determined by proton translocation studies and freeze-fracture electron microscopy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
321-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3138947-Cell Fractionation,
pubmed-meshheading:3138947-Chlorophyll,
pubmed-meshheading:3138947-Cyanobacteria,
pubmed-meshheading:3138947-Freeze Fracturing,
pubmed-meshheading:3138947-Hydrogen-Ion Concentration,
pubmed-meshheading:3138947-Intracellular Membranes,
pubmed-meshheading:3138947-Light-Harvesting Protein Complexes,
pubmed-meshheading:3138947-Magnesium,
pubmed-meshheading:3138947-Microscopy, Electron,
pubmed-meshheading:3138947-Photosynthetic Reaction Center Complex Proteins,
pubmed-meshheading:3138947-Plant Proteins,
pubmed-meshheading:3138947-Subcellular Fractions,
pubmed-meshheading:3138947-Surface Properties
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pubmed:year |
1988
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pubmed:articleTitle |
Generation and isolation of cyanobacterial inside-out thylakoid vesicles.
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pubmed:affiliation |
Department of Biochemistry, Arrhenius Laboratories, University of Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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