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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1988-9-7
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pubmed:abstractText |
Paramyosin (Sm97), a 97-kDa myofibrillar protein identified by the unusually monospecific antibody response induced by intradermal vaccination of mice with a complex soluble worm antigen preparation (SWAP) of adult Schistosoma mansoni administered with bacillus Calmette-Guérin (BCG), was purified and tested for its capacity to protect mice against challenge infection. When administered intradermally with BCG at total doses of only 4-40 micrograms per mouse, both the native molecule and a recombinant expression product containing approximately 50% of the whole protein were found to confer significant resistance (26-33%) against challenge infection, while 2 mg of unfractionated SWAP was required to induce similar levels of protection. In addition, paramyosin was shown to stimulate T lymphocytes from vaccinated mice to produce lymphokines [e.g., gamma interferon (IFN-gamma)] that activate macrophages to kill schistosomula. Neither schistosome myosin nor a heterologous paramyosin from a different invertebrate genus were protective, indicating a requirement for specific epitopes in the immunization. That the protection induced by paramyosin involves a T-cell-mediated mechanism was supported by the failure of anti-paramyosin antibodies to passively transfer significant resistance to infection to recipient mice. Lymphocytes from mice vaccinated with paramyosin were found to produce IFN-gamma in response to living schistosomula, suggesting that during challenge infection of vaccinated hosts, paramyosin (a nonsurface antigen) may elicit a protective T-cell response as a consequence of its release from migrating parasite larvae. Paramyosin-depleted SWAP was also found to be protective as well as stimulatory for T lymphocytes from SWAP-vaccinated mice, indicating that other antigens in this preparation may have immunoprophylactic potential. In summary, these results (i) suggest that the induction of T-cell-dependent cell-mediated immunity against soluble nonsurface antigens may be an effective strategy for immunization against multicellular parasites and (ii) in the case of schistosomes, identify paramyosin as a candidate vaccine immunogen in this category.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-140764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-2431037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-2578525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3084645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3084646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3084647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3094144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3106483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3119723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3120134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3127798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3284744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3317823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3534695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3892433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-3980998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-6276213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-6431002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-6696190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3135553-6973741
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5678-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3135553-Animals,
pubmed-meshheading:3135553-Antigens, Helminth,
pubmed-meshheading:3135553-Female,
pubmed-meshheading:3135553-Immunity, Cellular,
pubmed-meshheading:3135553-Immunization, Passive,
pubmed-meshheading:3135553-Injections, Intradermal,
pubmed-meshheading:3135553-Interferon-gamma,
pubmed-meshheading:3135553-Macrophages,
pubmed-meshheading:3135553-Mice,
pubmed-meshheading:3135553-Mycobacterium bovis,
pubmed-meshheading:3135553-Schistosoma mansoni,
pubmed-meshheading:3135553-Schistosomiasis mansoni,
pubmed-meshheading:3135553-T-Lymphocytes,
pubmed-meshheading:3135553-Tropomyosin,
pubmed-meshheading:3135553-Vaccination,
pubmed-meshheading:3135553-Vaccines,
pubmed-meshheading:3135553-Vaccines, Synthetic
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pubmed:year |
1988
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pubmed:articleTitle |
Induction of protective immunity against Schistosoma mansoni by vaccination with schistosome paramyosin (Sm97), a nonsurface parasite antigen.
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pubmed:affiliation |
Immunology and Cell Biology, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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