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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1988-7-25
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pubmed:abstractText |
Transfection of deleted forms of the human interleukin 2 receptor alpha subunit (IL-2R alpha; also called CD25 or Tac antigen) gene (IL2RA) promoter revealed a requirement for sequences 3' of base -317 for phytohemagglutinin- and phorbol 12-myristate 13-acetate (PMA)-induced promoter activation in CD4+ Jurkat T cells. In contrast, sequences 3' of base -271 were sufficient for promoter induction in CD4-/CD8- YT-1 T cells or Jurkat cells expressing the transactivator protein (tat-I) of human T-cell lymphotropic virus type I (HTLV-I). Gel retardation assays revealed that nuclear extracts from induced, but not uninduced, Jurkat and YT-1 cells mediated the formation of two specific DNA-protein complexes with oligonucleotides spanning the region of the IL2RA promoter from position -291 to -245, which contains two imperfect direct repeats (IDRs). Consistent with the different 5' sequence requirements for promoter activation in Jurkat and YT-1 cells, oligonucleotides corresponding to the region from -267 to -243 (downstream IDR and flanking region) formed only one complex with induced Jurkat extracts but two complexes with induced YT-1 extracts. Oligonucleotides containing the region of the IL2RA promoter from -293 to -270 (upstream IDR and flanking region) failed to bind protein in either cell type. In further support of the biological significance of these DNA-protein interactions, the IL2RA oligonucleotide from -291 to -245 proved to be sufficient in either orientation to confer PMA inducibility to the mitogen-insensitive thymidine kinase gene promoter in Jurkat cells. Together, these findings suggest that the interaction of inducible DNA binding proteins with the IL2RA promoter between bases -291 and -245 plays an important role in mitogen-induced changes in the transcriptional activity of this receptor gene. Furthermore, the requisite 5' sequences appear to differ in T cells depending upon the nature of the activation signal and perhaps the stage of cellular maturation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-2413364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-2418464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-2437143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-2578514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-2938741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-2996141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3005464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3011033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3023889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3024966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3026643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3029959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3030566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3031040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3031660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3037548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3040856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3091480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3095922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3098894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3099289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3108674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3116143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-3116145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-4041012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6088625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6092466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6098465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6269744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6313230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6443342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6606011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6815536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3132714-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4468-72
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3132714-Base Sequence,
pubmed-meshheading:3132714-Chromosome Deletion,
pubmed-meshheading:3132714-Gene Expression Regulation,
pubmed-meshheading:3132714-Genes,
pubmed-meshheading:3132714-Genetic Vectors,
pubmed-meshheading:3132714-Humans,
pubmed-meshheading:3132714-Interleukin-2,
pubmed-meshheading:3132714-Molecular Sequence Data,
pubmed-meshheading:3132714-Nuclear Proteins,
pubmed-meshheading:3132714-Promoter Regions, Genetic,
pubmed-meshheading:3132714-Receptors, Immunologic,
pubmed-meshheading:3132714-Receptors, Interleukin-2,
pubmed-meshheading:3132714-Transcription, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
Regulation of interleukin 2 receptor alpha subunit (Tac or CD25 antigen) gene expression: binding of inducible nuclear proteins to discrete promoter sequences correlates with transcriptional activation.
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pubmed:affiliation |
Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham 27710.
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pubmed:publicationType |
Journal Article
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