|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009017,
umls-concept:C0025248,
umls-concept:C0086418,
umls-concept:C0205163,
umls-concept:C0230812,
umls-concept:C0332232,
umls-concept:C0392223,
umls-concept:C0679058,
umls-concept:C1547699,
umls-concept:C1705111,
umls-concept:C2700640
|
|
pubmed:issue |
11
|
|
pubmed:dateCreated |
1988-7-8
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Although several lysosomal membrane glycoproteins have been characterized by using specific antibodies, none of the studies so far elucidated the amino acid sequence of a lysosomal membrane glycoprotein. Here we describe cDNA clones encoding for one of the lysosome-associated membrane proteins with apparent Mr approximately equal to 120,000, lamp A. The amino acid sequence based on the fully coded cDNA shows that as many as 18 potential N-glycosylation sites can be found in the total of 385 amino acid residues. The results obtained by endoglycosidase F digestion support the conclusion that this glycoprotein contains 18 N-glycans. These N-glycosylation sites are clustered in two domains; one contains 10 and the other contains 8 N-glycosylation sites. These domains are separated by a (proline-serine)-rich region that has a distinct homology to the IgA hinge structure. The first N-glycosylated domain is elongated to a potential leader peptide toward the NH2-terminal end. The second N-glycosylated domain, on the other hand, is connected to a putative transmembrane portion consisting of hydrophobic amino acids. This segment, in turn, is elongated to a short cytoplasmic segment composed of 11 amino acid residues at the COOH-terminal end.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2409098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2441386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2582407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-28524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-286295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2871029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2943218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2943740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2943741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2945825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2951738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2954652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-2954653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3315809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3456608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3477806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3484330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3782140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3890942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3905822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3916320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3922993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-3943125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-4009718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-4373463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-6087149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-6298247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-6319122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-6328312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-6337626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-6715323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-6852022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-7035466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3131762-7190150
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
85
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3743-7
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3131762-Amino Acid Sequence,
pubmed-meshheading:3131762-Antigens, CD,
pubmed-meshheading:3131762-Base Sequence,
pubmed-meshheading:3131762-Cloning, Molecular,
pubmed-meshheading:3131762-DNA,
pubmed-meshheading:3131762-Humans,
pubmed-meshheading:3131762-Immunoglobulin alpha-Chains,
pubmed-meshheading:3131762-Lysosome-Associated Membrane Glycoproteins,
pubmed-meshheading:3131762-Lysosomes,
pubmed-meshheading:3131762-Membrane Glycoproteins,
pubmed-meshheading:3131762-Molecular Sequence Data,
pubmed-meshheading:3131762-Molecular Weight
|
|
pubmed:year |
1988
|
|
pubmed:articleTitle |
Molecular cloning of cDNAs encoding lamp A, a human lysosomal membrane glycoprotein with apparent Mr approximately equal to 120,000.
|
|
pubmed:affiliation |
La Jolla Cancer Research Foundation, Cancer Research Center, CA 92037.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
