Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1988-6-28
|
| pubmed:abstractText |
Oligodendrocytes isolated from 18-19-day-old rat brain were homogenized in 0.32 M sucrose. The homogenate was centrifuged at 100,000 g for 50 min in a gradient containing 0.8, 1.05, and 1.3 M sucrose. Three discrete bands were obtained at the interfaces 0.32-0.8 (F1), 0.8-1.05 (F2), and 1.05-1.3 M (F3). The distribution of UDP-galactose:ceramide galactosyltransferase (CgalT) activity in each fraction was measured using liposomes containing normal fatty acid-containing ceramides (NFA-CgalT activity) or 2-hydroxy fatty acid-containing ceramides (HFA-CgalT activity). Although detection of both CgalT activities was possible in all fractions, HFA-CgalT activity was enriched in F1 and F2 fractions, which also showed an enrichment of Golgi and endoplasmic reticulum markers, respectively. It is interesting that NFA-CgalT activity was significantly enriched in the F2 fraction. These results suggest that hydroxylated and nonhydroxylated galactocerebrosides may be synthesized at different intracellular locations.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1887-93
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3131486-Animals,
pubmed-meshheading:3131486-Brain,
pubmed-meshheading:3131486-Cell Membrane,
pubmed-meshheading:3131486-Centrifugation, Density Gradient,
pubmed-meshheading:3131486-Endoplasmic Reticulum,
pubmed-meshheading:3131486-Female,
pubmed-meshheading:3131486-Galactosyltransferases,
pubmed-meshheading:3131486-Ganglioside Galactosyltransferase,
pubmed-meshheading:3131486-Golgi Apparatus,
pubmed-meshheading:3131486-Hydroxy Acids,
pubmed-meshheading:3131486-Immunoenzyme Techniques,
pubmed-meshheading:3131486-Male,
pubmed-meshheading:3131486-Microscopy, Electron,
pubmed-meshheading:3131486-Neuroglia,
pubmed-meshheading:3131486-Oligodendroglia,
pubmed-meshheading:3131486-Rats,
pubmed-meshheading:3131486-Rats, Inbred Strains,
pubmed-meshheading:3131486-Subcellular Fractions
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Subcellular distribution of UDP-galactose:ceramide galactosyltransferase in rat brain oligodendroglia.
|
| pubmed:affiliation |
Department of Neurology, Yale University School of Medicine, New Haven, CT 06510.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|