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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1988-6-22
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pubmed:abstractText |
Using two-dimensional immunoelectrophoresis and an antibody to alpha 2-antiplasmin, we assessed the plasmin generated in serum under different conditions as the plasmin-alpha 2-antiplasmin complex. Activation in serum of human [Glu1]plasminogen ([Glu1]Pg) by recombinant tissue plasminogen activator was inhibited by the normal serum levels of Cl- and was enhanced by physiological levels of fibrinogen in the presence or absence of Cl-. These results agree with the recognized ability of Cl- to induce a conformation in [Glu1]Pg less favorable for its activation than the conformation that results without Cl-. The enhancing effect of fibrinogen surpassed the inhibitory effect of Cl- over a wide range of recombinant tissue plasminogen activator concentrations in physiological serum. Lesser inhibition by Cl- was seen in a purified clot-lysis system, suggesting that [Glu1]Pg conformation when attached to soluble fibrin matrix was less affected by the anion. The data regarding the roles of circulating fibrinogen and Cl- in controlling the plasma level of activated [Glu1]Pg have important implications in thrombolytic therapy with recombinant tissue plasminogen activator.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-124594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-125178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-3105106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-3122757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-3456584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-3473492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-3680236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-4041421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-5044515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-6431630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-6450468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-6458494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-670204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3130631-7199524
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3595-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
Roles for chloride ion and fibrinogen in the activation of [Glu1]plasminogen in human plasma.
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pubmed:affiliation |
Department of Chemistry, University of Notre Dame, IN 46556.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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