Linked Life Data

3128982

Source:http://linkedlifedata.com/resource/pubmed/id/3128982

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1988-5-17
pubmed:abstractText
Functional and molecular probes are described which are useful to identify a 3-methylcholanthrene-inducible phenol UDP-glucuronosyltransferase (GTMC) from rat liver. Two different procedures for isolation of GTMC were compared, method 1 utilizing DEAE-Sepharose chromatography or method 2, chromatofocusing. Method 2 appeared to be superior in separating different isoenzymes. Subsequently the enzyme was purified by affinity chromatography on UDP-hexanolamine Sepharose. With both methods a protein was purified with a subunit Mr of 55,000, catalyzing glucuronidation of a variety of planar phenols and, in particular, of benzo(a)pyrene-3,6-quinol to its mono- and diglucuronide. Antibodies to GTMC recognized a polypeptide with a subunit Mr of 55,000 as the major 3-methylcholanthrene-inducible isoenzyme in rat liver microsomes. The described functional and molecular probes may help to differentiate GTMC from similar isoenzymes conjugating planar phenols and to elucidate its regulation and biological function.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1439-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Properties of a 3-methylcholanthrene-inducible phenol UDP-glucuronosyltransferase from rat liver.
pubmed:affiliation
Department of Pharmacology, University of Göttingen, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't