Linked Life Data

3128922

Source:http://linkedlifedata.com/resource/pubmed/id/3128922

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-5-24
pubmed:abstractText
8-Hydroxyquinoline-beta-D-glucoside has been shown to be a useful alternative substrate for the differentiation of members of the family Enterobacteriaceae based on the presence or absence of beta-glucosidase. In an identification protocol based on multipoint-inoculated plates 8-hydroxyquinoline-beta-D-glucoside when incorporated into an agar based medium supplemented with a ferric salt produced intense black pigmentation localised solely in the immediate vicinity of beta-glucosidase positive organisms. In contrast, using conventional aesculin plates, positive organisms produced a brown ferric-aesculetin complex, the diffusibility of which limited interpretation especially where large numbers of colonies were involved. Almost complete agreement was found when 1964 routine 'coliform' organisms were tested by both methods. The method described allows use of a 36 place 'minipin' inoculator without any danger of 'nearest neighbour' interference and constitutes a cost-effective method suitable for either manual or automatic reading.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0176-6724
pubmed:author
pubmed:issnType
Print
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
188-93
pubmed:dateRevised
2009-6-8
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Detection of specific bacterial enzymes by high contrast metal chelate formation. Part I. 8-Hydroxyquinoline-beta-D-glucoside, an alternative to aesculin in the differentiation of members of the family Enterobacteriaceae.
pubmed:affiliation
School of Chemical and Life Sciences, The Polytechnic, Newcastle upon Tyne, England, U.K.
pubmed:publicationType
Journal Article