Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-5-20
pubmed:abstractText
We have used DNA-mediated gene transfer to express HLA class II molecules in mouse L cells for serological, biochemical, and functional analysis. cDNA clones encoding the DR2 beta a and DR2 beta b products of the DR2Dw2 haplotype were subcloned into a mouse Moloney leukemia virus-based expression vector (pJ4) and transfected separately into mouse L cells together with a HLA-DR alpha/pJ4 construct. These transfectants have allowed differential analysis of the two DR2 beta products in a manner normally prohibited by the concomitant expression seen in B cells. Two-dimensional SDS-PAGE analysis of the transfectants defines the more acidic beta chain as the product of the DR2 beta a sequence, and the more basic chain as the product of the DR2 beta b sequence. The LDR2a transfectants present antigen efficiently to M.leprae-specific T cell clones and are capable of presenting synthetic peptide, 65-kD recombinant mycobacterial antigen and M.leprae. Of the DR2Dw2-restricted T cell clones we have tested, all use the DR2 beta a chain as their restriction element. Inhibition studies with mAbs demonstrate the dependence of presentation by the transfectant on class II and CD4, while mAbs against LFA-1, which substantially inhibit presentation by B-lymphoblastoid cell lines, do not inhibit transfectant presentation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-210957, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-23215, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-2409205, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-2431092, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-2435001, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-2578544, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-2885840, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-2995241, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3001928, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3108669, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3112582, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3159789, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3161627, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3485528, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3518745, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3525675, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3571980, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3875853, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3876392, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3876513, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3892536, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3894979, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3900200, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-3919144, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-392522, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6150235, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6156220, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6162228, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6178597, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6189259, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6192342, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6193218, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6193521, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6197354, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6198242, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6260957, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6294612, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6310611, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6339253, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6353531, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6427104, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6436430, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-667938, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6811517, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-6984012, http://linkedlifedata.com/resource/pubmed/commentcorrection/3128633-76894
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1442-58
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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