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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1988-5-20
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pubmed:abstractText |
Experimental anti-tubular basement membrane (anti-TBM) disease is an autoimmune interstitial nephritis elicited in susceptible rodents after immunization with renal tubular antigen. The nephritogenic antigen in the immunizing preparation is 3M-1, a 48,000 Mr noncollagenous glycoprotein. The hallmarks of the renal lesion are the presence of anti-TBM antibodies (anti-TBM-Ab) and a dense mononuclear cell infiltrate. The anti-TBM B cell repertoire in this disease was analyzed using a library of 22 anti-TBM mAbs generated in a prototypically susceptible Brown Norway rat. These anti-TBM mAbs were all demonstrated to be 3M-1 specific and their characterization formed the basis for the following observations: (a) The size of the anti-TBM B cell population is estimated at 58 distinct clones; (b) by competitive inhibition criteria, all anti-TBM mAbs recognize the same (or spatially close) epitope(s) on 3M-1. This focused recognition was maintained in spite of considerable variability in affinity. Epitopic dominance could also be demonstrated in human polyclonal anti-TBM antisera from a patient with anti-TBM disease; and (c) a crossreactive idiotype was documented, and antisera directed toward this set of variable region determinants was shown to be effective as a prophylactic regimen to abrogate disease, and as a therapeutic modality to arrest the progression of disease; (d) analysis of VH gene families suggested biased usage of Q52- and 7183-like families, although at least three gene families are used in the anti-TBM-Ab response. Thus, the anti-TBM B cell compartment in BN rats is moderately large, but is primarily focused to a single epitope on the nephritogenic antigen and is associated with a disease-modifying crossreactive idiotype.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-2427543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-2579142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3093628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3095832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3102673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3104460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-314961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3490512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3494096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3514674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3558816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-3882878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-4201411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-4211170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-447371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-4546826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-4623607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-489972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6092095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6195263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6223048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6231352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6528977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6768804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6772444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6790935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6826209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6976412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-6987327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-7057039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-7198664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-786519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-804130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3128629-99996
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Idiotypes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/anti-IgG,
http://linkedlifedata.com/resource/pubmed/chemical/renal tubular antigen
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
167
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1296-312
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3128629-Animals,
pubmed-meshheading:3128629-Antibodies, Anti-Idiotypic,
pubmed-meshheading:3128629-Antibodies, Monoclonal,
pubmed-meshheading:3128629-Antigens,
pubmed-meshheading:3128629-Autoantibodies,
pubmed-meshheading:3128629-Autoimmune Diseases,
pubmed-meshheading:3128629-B-Lymphocytes,
pubmed-meshheading:3128629-Basement Membrane,
pubmed-meshheading:3128629-Clone Cells,
pubmed-meshheading:3128629-Immunoglobulin G,
pubmed-meshheading:3128629-Immunoglobulin Heavy Chains,
pubmed-meshheading:3128629-Immunoglobulin Idiotypes,
pubmed-meshheading:3128629-Immunoglobulin Variable Region,
pubmed-meshheading:3128629-Kidney Tubules,
pubmed-meshheading:3128629-Nephritis, Interstitial,
pubmed-meshheading:3128629-Rats,
pubmed-meshheading:3128629-Rats, Inbred BN,
pubmed-meshheading:3128629-Rats, Inbred Lew
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pubmed:year |
1988
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pubmed:articleTitle |
Clonotypic heterogeneity in experimental interstitial nephritis. Restricted specificity of the anti-tubular basement membrane B cell repertoire is associated with a disease-modifying crossreactive idiotype.
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