| pubmed-article:3128548 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3128548 | lifeskim:mentions | umls-concept:C0032143 | lld:lifeskim |
| pubmed-article:3128548 | lifeskim:mentions | umls-concept:C0014261 | lld:lifeskim |
| pubmed-article:3128548 | lifeskim:mentions | umls-concept:C0030190 | lld:lifeskim |
| pubmed-article:3128548 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:3128548 | lifeskim:mentions | umls-concept:C1511613 | lld:lifeskim |
| pubmed-article:3128548 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:3128548 | pubmed:dateCreated | 1988-5-18 | lld:pubmed |
| pubmed-article:3128548 | pubmed:abstractText | Regulation of the fibrinolytic system of cultured human umbilical vein endothelial cells (HUVECs) by recombinant interleukin 1 beta (rIL-1 beta) and tumor necrosis factor alpha (rTNF alpha) was investigated. Functional and immunologic assays indicated that both cytokines decreased HUVEC tissue-type plasminogen activator (tPA) and increased type 1 plasminogen activator inhibitor (PAI-1) in a dose- and time-dependent manner. Maximal effects (50% decrease in tPA antigen; 300-400% increase in PAI-1 activity) were achieved with 2.5 units/ml rIL-1 beta and 200 units/ml rTNF alpha. Combinations of rIL-1 beta and rTNF alpha were not additive at these maximal concentrations. After a 24-h pretreatment with rIL-1 beta, HUVECs secreted tPA at one-quarter of the rate of control cells and released PAI-1 at a rate that was 5-fold higher than controls. Neither the basal rate of PAI-1 release nor the increased rate of release of PAI-1 in response to rIL-1 beta was affected by subsequently treating the cells with secretagogues (e.g. phorbol myristate acetate) suggesting that PAI-1 is not contained within a rapidly releasable, intracellular storage pool. Northern blot analysis using a PAI-1 cDNA probe indicated that the cytokines increased the steady-state levels of the 3.2- and 2.3-kb PAI-1 mRNA species, but with a preferential increase in the larger mRNA form. The fact that both rIL-1 beta and rTNF alpha act in a similar manner strengthens the hypothesis that the local development of inflammatory/immune processes could reduce endothelial fibrinolytic activity. | lld:pubmed |
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| pubmed-article:3128548 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3128548 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3128548 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3128548 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:3128548 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:3128548 | pubmed:author | pubmed-author:LoskutoffD... | lld:pubmed |
| pubmed-article:3128548 | pubmed:author | pubmed-author:GimbroneM... | lld:pubmed |
| pubmed-article:3128548 | pubmed:author | pubmed-author:SchleefR RRR | lld:pubmed |
| pubmed-article:3128548 | pubmed:author | pubmed-author:BevilacquaM... | lld:pubmed |
| pubmed-article:3128548 | pubmed:author | pubmed-author:SawdeyMM | lld:pubmed |
| pubmed-article:3128548 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3128548 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:3128548 | pubmed:volume | 263 | lld:pubmed |
| pubmed-article:3128548 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3128548 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3128548 | pubmed:pagination | 5797-803 | lld:pubmed |
| pubmed-article:3128548 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:3128548 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3128548 | pubmed:articleTitle | Cytokine activation of vascular endothelium. Effects on tissue-type plasminogen activator and type 1 plasminogen activator inhibitor. | lld:pubmed |
| pubmed-article:3128548 | pubmed:affiliation | Research Institute of Scripps Clinic, Department of Immunology, La Jolla, California 92037. | lld:pubmed |
| pubmed-article:3128548 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3128548 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:3128548 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:3128548 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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