Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
|
pubmed:dateCreated |
1988-5-23
|
pubmed:abstractText |
The murine B cell lymphoma 70Z/3 is a tumor line that resembles an early B cell. It responds to lipopolysaccharide by synthesizing kappa light chains, resulting in the appearance of surface IgM. We now demonstrate that this effect is triggered by the lipid A domain of lipopolysaccharide since a defined tetraacyl disaccharide 1,4'-bisphosphate precursor of mature lipid A, designated lipid IVA (Raetz, C. R. H., Purcell, S., Meyer, M. V., Qureshi, N., and Takayama, K. (1985) J. Biol. Chem. 260, 16080-16088), is fully active in stimulating kappa chain mRNA synthesis. In contrast, the monosaccharide lipid A precursor, lipid X, shows only slight activity; but a large excess of lipid X appears to complete with lipid IVA, partially blocking its effects. Mutants of the 70Z/3 line that are unresponsive to lipopolysaccharide also fail to respond to lipid IVA. The somatic cell system described here, coupled with the use of chemically defined agonists and antagonists, offers a new approach to understanding the early events in lipopolysaccharide/animal cell interactions.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin kappa-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/lipid A precursors, bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/lipid X
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0021-9258
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
263
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
5098-103
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:3128535-Animals,
pubmed-meshheading:3128535-B-Lymphocytes,
pubmed-meshheading:3128535-Escherichia coli,
pubmed-meshheading:3128535-Glycolipids,
pubmed-meshheading:3128535-Immunoglobulin kappa-Chains,
pubmed-meshheading:3128535-Lipid A,
pubmed-meshheading:3128535-Lymphoma,
pubmed-meshheading:3128535-Mice,
pubmed-meshheading:3128535-RNA, Messenger,
pubmed-meshheading:3128535-Salmonella typhimurium,
pubmed-meshheading:3128535-Tumor Cells, Cultured
|
pubmed:year |
1988
|
pubmed:articleTitle |
Induction of kappa light chain synthesis in 70Z/3 B lymphoma cells by chemically defined lipid A precursors.
|
pubmed:affiliation |
Department of Genetics, University of Washington, Seattle 98195.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|