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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1988-5-12
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pubmed:abstractText |
The effects of ecdysone, the steroid molting hormone of arthropods, are of considerable interest both to insect physiologists and to those studying steroid-regulated gene expression. Yet progress in understanding ecdysone receptors has been inhibited by the lack of a suitable highly radioactive hormone analog with high affinity for the receptor. Here we report that the synthetic ecdysteroid 26-iodoponasterone A is one of the most active ecdysones known, inducing half-maximal morphological transformation in Drosophila Kc167 cells when present at 0.5 nM. 26-[125I]Iodoponasterone A can be prepared at a specific activity of 2175 Ci/mmol (1 Ci = 37 GBq) by reaction of the precursor 26-mesylinokosterone with carrier-free Na125I. The radiolabeled material binds to Kc167 cell ecdysone receptors specifically and with affinity (Kd ca. 3.8 X 10(-10) M). Thus, 26-[125I]iodoponasterone A appears to be a superior radioligand for ecdysone receptors on grounds both of affinity and of specific activity. Its ready availability should greatly facilitate studies of these receptors.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-110630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-1273900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-16453674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-16453691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-16578683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-16593141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-216008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-216009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-2431935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-3007239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-3007512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-3025658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-3086161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-3091364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-3097323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-3102749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-3104771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-4394304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-4628122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-472733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-4745661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-4773932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-4885833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-4942193,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-5768877,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-6699012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-6707018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-6751563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-6800658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-6804465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-6820371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127825-7091684
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2096-100
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
26-[125I]iodoponasterone A is a potent ecdysone and a sensitive radioligand for ecdysone receptors.
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pubmed:affiliation |
Department of Biology, Indiana University, Bloomington 47405.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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