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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-5-12
pubmed:databankReference
pubmed:abstractText
An inducible resistance determinant, ermSF, from the tylosin producer Streptomyces fradiae NRRL 2338 has been cloned, sequenced, and shown to confer inducible macrolide-lincosamide-streptogramin B resistance when transferred to Streptomyces griseofuscus NRRL 23916. From mapping studies with S1 nuclease to locate the site of transcription initiation, the ermSF message contains a 385-nucleotide 5' leader sequence upstream from the 960-nucleotide major open reading frame that encodes the resistance determinant. On the basis of the potential secondary structure that the ermSF leader can assume, a translational attenuation model similar to that for ermC is proposed. The model is supported by mutational analysis involving deletions in the proposed attenuator. By analysis with restriction endonucleases, ermSF is indistinguishable from the tlrA gene described by Birmingham et al. (V. A. Birmingham, K. L. Cox, J. L. Larson, S. E. Fishman, C. L. Hershberger, and E. T. Seno, Mol. Gen. Genet. 204:532-539, 1986) which comprises one of at least three genes from S. fradiae that can confer tylosin resistance when subcloned into S. griseofuscus. When tested for inducibility, ermSF appears to be strongly induced by erythromycin, but not by tylosin.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-2579378, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-2985541, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-2998943, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-3012613, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-3020383, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-3025178, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-3036668, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-3297922, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-3934045, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-3996185, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-4043733, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-4224220, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-438126, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-4397638, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-440414, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-4598299, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-5279527, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6096212, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6162157, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6163133, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6163765, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6195156, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6203682, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6246368, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6298066, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6304321, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6317655, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6406429, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6429477, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6657780, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6780509, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6810064, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6833128, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-6938954, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-731205, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-881736, http://linkedlifedata.com/resource/pubmed/commentcorrection/3127381-922889
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1800-11
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:3127381-Anti-Bacterial Agents, pubmed-meshheading:3127381-Mutation, pubmed-meshheading:3127381-Streptomyces, pubmed-meshheading:3127381-Erythromycin, pubmed-meshheading:3127381-Leucomycins, pubmed-meshheading:3127381-Drug Resistance, Microbial, pubmed-meshheading:3127381-DNA, Bacterial, pubmed-meshheading:3127381-Base Sequence, pubmed-meshheading:3127381-Genes, Bacterial, pubmed-meshheading:3127381-Protein Biosynthesis, pubmed-meshheading:3127381-Amino Acid Sequence, pubmed-meshheading:3127381-Phenotype, pubmed-meshheading:3127381-Virginiamycin, pubmed-meshheading:3127381-Molecular Sequence Data, pubmed-meshheading:3127381-Transcription, Genetic, pubmed-meshheading:3127381-Methyltransferases, pubmed-meshheading:3127381-Cloning, Molecular, pubmed-meshheading:3127381-Gene Expression Regulation, pubmed-meshheading:3127381-Macrolides
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