Linked Life Data

3126374

Source:http://linkedlifedata.com/resource/pubmed/id/3126374

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1988-4-19
pubmed:abstractText
In order to define the early transient responses of peptic cells to stimulation, we have modified the acidified hemoglobin substrate digestion method for pepsin to provide a very sensitive, linear (10-250 ng/ml), reproducible, inexpensive (less than 3 cents/sample) and simple semi-automated assay. Using a specially designed perifusion chamber, this method was used to accurately measure secretion at 1 min intervals from approximately 3 mg of isolated peptic glands from the esophageal peptic organ of R. catesbeiana, containing approximately 150 micrograms pepsinogen. Responses to carbachol applied for 1, 2 and 4 min could be described in discrete 1 min intervals. Secretion stimulated by carbachol (1-2 min) peaked at 200-300% of basal and upon withdrawal decayed with t1/2 approximately 3 min. Atropine added to continuous carbachol stimulation inhibited secretion with t1/2 approximately 4 min, indicating rapid metabolism of activated messengers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0024-3205
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1237-44
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Pepsinogen secretion from perifused frog peptic glands: rapid transients detected with a modified pepsin assay.
pubmed:affiliation
Division of Gastroenterology, University Station, Birmingham, AL 35294.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't