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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1988-3-14
|
| pubmed:abstractText |
The effect of pKM101 plasmid on repair of single-strand breaks in DNA induced by 60Co-gamma irradiation in E. coli K12 AB1157 (wild type) and in its recA- and recB- mutant cells was studied by alkaline sucrose gradient sedimentation method. For quantitative analysis of sedimentation profiles we calculated the S1/2 values described by Veatch and Okada. The S1/2 values of unirradiated cells were 21.10, and after 200 Gray irradiation 11.35, due to the original incidence of single-strand breaks. The presence of pKM101 did not influence these values in either cases. This means that pKM101 had no effect on the rise of single-strand breaks in DNA. During a post-irradiation incubation period at 37 degrees C for 60 min the S1/2 value of the wild type strain increased from 11.35 to 19.22, that of the recB- from 11.50 to 15.23, while the S1/2 value of the recA- mutant did not change owing to the lack of repair of single-strand breaks. pKM101 plasmid markedly increased the S1/2 value in wild type strain and in recB- mutant, while it had no effect on S1/2 in recA- cells, during this post-irradiation incubation period. Thus the effect of pKM101 on the repair of single-strand breaks in DNA proved to be dependent on recA+ genotype. Nalidixic acid at 100 micrograms/ml concentration inhibited the repair of single-strand breaks in both wild type and recB- mutant cells harbouring pKM101 plasmid.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0237-6261
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
85-97
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:3124425-Centrifugation, Density Gradient,
pubmed-meshheading:3124425-Cobalt Radioisotopes,
pubmed-meshheading:3124425-DNA, Bacterial,
pubmed-meshheading:3124425-DNA Repair,
pubmed-meshheading:3124425-Escherichia coli,
pubmed-meshheading:3124425-Mutation,
pubmed-meshheading:3124425-Nalidixic Acid,
pubmed-meshheading:3124425-Plasmids
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Effect of the pKM101 plasmid on the repair of single-strand breaks in DNA induced by ionizing irradiation in Escherichia coli.
|
| pubmed:affiliation |
Central Research Laboratory, University Medical School, Debrecen, Hungary.
|
| pubmed:publicationType |
Journal Article
|