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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0018270,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0021745,
umls-concept:C0036667,
umls-concept:C0042764,
umls-concept:C0086418,
umls-concept:C0312418,
umls-concept:C0334227,
umls-concept:C0684249,
umls-concept:C1448177,
umls-concept:C1533691,
umls-concept:C1948058
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pubmed:issue |
11
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pubmed:dateCreated |
1988-2-23
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pubmed:abstractText |
The colony-inhibitory effects of recombinant human tumor necrosis factor (rH-TNF) and recombinant human interferon-gamma (rH-IFN-gamma) were evaluated in four human lung cancer cell lines and their cisplatin-resistant sublines. The cell lines tested were PC-7 and PC-9 (adenocarcinoma), H69 and N231 (small cell lung cancer) and four cisplatin-resistant sublines, PC-7/1.0, PC-9/0.5, H69/0.2 and N231/0.2, which were 20.0, 7.1, 4.8 and 8.4 fold resistant to cisplatin, respectively, compared to the respective parental cell line in terms of IC50 in a soft agar colony assay. All parental cell lines were resistant to rH-TNF and rH-IFN-gamma, alone or in combination. However, two resistant sublines showed sensitivity to rH-TNF and rH-IFN-gamma. Colony formation by PC-9/0.5 was significantly inhibited, in the absence or presence of cisplatin, by 10(2) U/ml of rH-TNF (less than 50% of control) and the inhibition was synergistic with that produced by 10(3) or 10(4) U/ml of rH-IFN-gamma. RH-IFN-gamma inhibited the colony formation of H69/0.2 only at the highest concentration tested (10(4) U/ml) (less than 50% of control) and the combined effect with rH-TNF was additive. These results suggest that rH-TNF and rH-IFN-gamma may have some potential in overcoming cisplatin resistance by virtue of collateral sensitivity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0910-5050
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1274-80
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3121562-Cisplatin,
pubmed-meshheading:3121562-Drug Resistance,
pubmed-meshheading:3121562-Drug Synergism,
pubmed-meshheading:3121562-Humans,
pubmed-meshheading:3121562-Interferon-gamma,
pubmed-meshheading:3121562-Lung Neoplasms,
pubmed-meshheading:3121562-Recombinant Proteins,
pubmed-meshheading:3121562-Tumor Cells, Cultured,
pubmed-meshheading:3121562-Tumor Necrosis Factor-alpha
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pubmed:year |
1987
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pubmed:articleTitle |
In vitro growth inhibition of cisplatin-resistant human lung cancer cell lines by recombinant human tumor necrosis factor and/or recombinant human interferon-gamma by virtue of collateral sensitivity.
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pubmed:affiliation |
Department of Internal Medicine, Korea Cancer Center Hospital, Seoul.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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