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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1987-12-21
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pubmed:abstractText |
In vitro immortalized cell lines with the morphology and phenotype of mature macrophages (M phi) have been generated by infecting freshly isolated bone marrow cells from C3H/HeJ mice with a recombinant retrovirus carrying v-raf and v-myc oncogenes. All of the clones obtained had M phi-like phenotypes, and one such clone, GG2EE, has been compared to normal M phi to ascertain the effects of immortalization on the expression of the biological functions of the lines. GG2EE cells expressed cytotoxic activity against L5178Y, P815 or RL male 1 target cells in response to stimulation with interferon-gamma (IFN-gamma) and heat-killed Listeria monocytogenes; in contrast, they failed to kill YAC-1 target cells. GG2EE cells did not constitutively express I-A or I-E antigens; nevertheless, I region-coded antigens could be induced by IFN-gamma treatment. GG2EE cells produced interleukin 1 upon stimulation with a T cell-derived lymphokine; they were weakly phagocytic, yet became highly phagocytic following IFN-gamma treatment. Since c-fos mRNA is augmented in peritoneal exudate M phi by protein kinase C activators but not by IFN-gamma, we evaluated the effects of calcium ionophore, phorbol myristate acetate, L-alpha-1-oleoyl-2-acetoyl-sn-3 glycerol (OAG) and IFN-gamma on the levels of c-fos mRNA in GG2EE cells. We found that calcium ionophore, PMA and OAG stimulation enhanced the expression of c-fos mRNA, but IFN-gamma treatment did not. The kinetics of c-fos induction in GG2EE cells were also comparable to those observed in peritoneal exudate M phi. Overall, the GG2EE cell line has the same biological properties as normal tissue M phi. Because it is capable of both constitutive and inducible M phi-like functions, this cell line provides a valuable tool for studying the molecular mechanisms controlling induction and/or expression of biological activities in M phi. It is striking that a cell line immortalized in vitro by two oncogenes, v-raf and v-myc, behaves, according to the criteria mentioned above, like a normal M phi population.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-2980
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1491-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3119352-Animals,
pubmed-meshheading:3119352-Cell Line,
pubmed-meshheading:3119352-Cell Transformation, Viral,
pubmed-meshheading:3119352-Cytotoxicity, Immunologic,
pubmed-meshheading:3119352-Female,
pubmed-meshheading:3119352-Histocompatibility Antigens Class II,
pubmed-meshheading:3119352-Interferon-gamma,
pubmed-meshheading:3119352-Interleukin-1,
pubmed-meshheading:3119352-Macrophages,
pubmed-meshheading:3119352-Mice,
pubmed-meshheading:3119352-Mice, Inbred C3H,
pubmed-meshheading:3119352-Oncogenes,
pubmed-meshheading:3119352-Phagocytosis,
pubmed-meshheading:3119352-Phenotype,
pubmed-meshheading:3119352-RNA, Messenger
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pubmed:year |
1987
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pubmed:articleTitle |
A murine macrophage cell line, immortalized by v-raf and v-myc oncogenes, exhibits normal macrophage functions.
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pubmed:affiliation |
Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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