rdf:type |
|
lifeskim:mentions |
umls-concept:C0001699,
umls-concept:C0024432,
umls-concept:C0033268,
umls-concept:C0205470,
umls-concept:C0431085,
umls-concept:C0441655,
umls-concept:C0596402,
umls-concept:C1511636,
umls-concept:C1521761,
umls-concept:C1555707,
umls-concept:C1705851,
umls-concept:C1879547,
umls-concept:C2752151,
umls-concept:C2828366
|
pubmed:issue |
4
|
pubmed:dateCreated |
1988-1-4
|
pubmed:abstractText |
RU-41740, a glycoprotein extract from Klebsiella pneumoniae, is an immunomodulating agent with a broad spectrum of activities. It enhances several macrophage functions, including interleukin-1 (IL-1) secretion. Present data show that RU-41740 was able to promote murine macrophage cytotoxicity against tumour cells. A soluble cytotoxic factor (CF) was found in supernatants from macrophage cultures stimulated with RU-41740. These supernatants were shown to contain IL-1. CF was detected on L-929 cells sensitized by actinomycin D. CF was analysed on the basis of MW and pHi: after gel filtration on "Ultrogel Aca54", a single peak of CF was found in the range of 50-60 Kd and was then distinct from IL-1, which was eluted at 15 Kd. After chromatofocusing, CF was found in a narrow peak of pH 4.8. CF was detected in supernatants 2 h after macrophage stimulation by RU-41740, and its release was abolished by pretreatment of macrophages with cycloheximide (2 micrograms/ml). CF described here shares several properties with tumour necrosis factor (TNF).
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:issn |
0769-2625
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
138
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
571-84
|
pubmed:dateRevised |
2007-7-24
|
pubmed:meshHeading |
pubmed-meshheading:3118904-Animals,
pubmed-meshheading:3118904-Bacterial Proteins,
pubmed-meshheading:3118904-Cycloheximide,
pubmed-meshheading:3118904-Cytotoxicity, Immunologic,
pubmed-meshheading:3118904-Female,
pubmed-meshheading:3118904-Glycoproteins,
pubmed-meshheading:3118904-Humans,
pubmed-meshheading:3118904-Indomethacin,
pubmed-meshheading:3118904-Interleukin-1,
pubmed-meshheading:3118904-Klebsiella pneumoniae,
pubmed-meshheading:3118904-Lymphocyte Activation,
pubmed-meshheading:3118904-Macrophages,
pubmed-meshheading:3118904-Mast-Cell Sarcoma,
pubmed-meshheading:3118904-Mice,
pubmed-meshheading:3118904-Mice, Inbred C3H,
pubmed-meshheading:3118904-Mice, Inbred DBA,
pubmed-meshheading:3118904-Tumor Necrosis Factor-alpha
|
pubmed:articleTitle |
Immunological activities of RU-41740, a glycoproteic extract from Klebsiella pneumoniae. II.--Activation of macrophage cytotoxicity against tumour cells and production of a cytotoxic factor.
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pubmed:affiliation |
Laboratoire de Microbiologie, UFR de Médecine Paris Ouest, Garches, France.
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pubmed:publicationType |
Journal Article
|