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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1987-11-25
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pubmed:abstractText |
The effects of recombinant human gamma interferon (rHuIFN-gamma; two identical monomers of 140 residues in length) and of two re-engineered C-terminal variants, rHuIFN-gamma Tetra-Ser (residues 129 to 132 replaced by serine) and rHuIFN-gamma 125 (two identical monomers of 125 residues each with the last 14 residues plus an additional alanine from the C terminus deleted), were compared in terms of several in vitro biological activities. By using three different human cell lines (HeLa 229, HEp-2, and A549), the interferons were tested for their ability to inhibit: (i) growth of Chlamydia trachomatis; (ii) replication of encephalomyocarditis virus; and (iii) cell growth. rHuIFN-gamma restricted the growth of chlamydiae to 50% of the non-IFN-treated control at concentrations ranging from 0.01 to 0.05 ng/ml, depending on the cell type assayed. One of the modified proteins, rHuIFN-gamma Tetra-Ser, also decreased the growth of chlamydiae, but it required a concentration of approximately 0.5 ng/ml to produce 50% inhibition. rHuIFN-gamma 125 had the lowest antichlamydial activity of the three IFN-gamma variants tested; concentrations of 1 to 20 ng/ml were needed to reduce the growth of C. trachomatis to 50% of that of the control. The relative antiviral and antiproliferative activities of the three IFN-gamma preparations paralleled their antichlamydial activities in these three cell lines. The antiencephalomyocarditis virus activities of rHuIFN-gamma Tetra-Ser and rHuIFN-gamma 125 were reduced by approximately 10-fold and 10(2)- to 10(3)-fold, respectively, compared with the antiviral activity of rHuIFN-gamma. Proliferation of the three cell lines was restricted to approximately 50% of the control with 0.5 to 10 ng of rHuIFN-gamma per ml. Inhibition of cell growth by rHuIFN-gamma Tetra-Ser was significant only at concentrations equal to or greater than 30 ng/ml, and the rHuIFN-gamma 125 variant did not significantly decrease the growth of any of the three cell lines at the concentrations tested. These results suggest that the C-terminal portion of rHuIFN-gamma is critical for maintaining the conformation necessary for inducing the antichlamydial, antiviral, and antiproliferative activities of the molecule.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-13986422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-16558062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-17788296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-17838106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-3087976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-3933006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-3934279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-3936412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-3972450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-5686006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6172996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6173769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6175461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6225933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6307876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6312213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6313807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6417024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6427223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-6479293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-7060724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3117689-942051
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-9567
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2727-33
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:3117689-Amino Acid Sequence,
pubmed-meshheading:3117689-Antiviral Agents,
pubmed-meshheading:3117689-Cell Division,
pubmed-meshheading:3117689-Chlamydia,
pubmed-meshheading:3117689-HeLa Cells,
pubmed-meshheading:3117689-Humans,
pubmed-meshheading:3117689-Interferon-gamma,
pubmed-meshheading:3117689-Molecular Weight,
pubmed-meshheading:3117689-Recombinant Proteins,
pubmed-meshheading:3117689-Structure-Activity Relationship,
pubmed-meshheading:3117689-Tumor Cells, Cultured
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pubmed:year |
1987
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pubmed:articleTitle |
The antichlamydial, antiviral, and antiproliferative activities of human gamma interferon are dependent on the integrity of the C terminus of the interferon molecule.
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pubmed:affiliation |
Department of Pathology, University of California, Irvine 92717.
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pubmed:publicationType |
Journal Article
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