Linked Life Data

3116711

Source:http://linkedlifedata.com/resource/pubmed/id/3116711

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-10-30
pubmed:abstractText
We developed new monoclonal antibodies (NNKY1-32, NNKY2-6, NNKY2-18) that react with a determinant specific to the GPIIb-IIIa complex, and studied the distribution of GPIIb/IIIa on the platelet surface in vivo and some factors that influence the structure of GPIIb/IIIa using flow cytometry. 1. The surface of large platelets is richer in GPIIb/IIIa than that of small platelets. 2. Incubation of intact platelets with EDTA at 37 degrees C causes progressive dissociation of GPIIb-IIIa complexes, and this influence is marked in small platelets. 3. Addition of Ca2+ or Mg2+ to dissociated GPIIb-IIIa complexes causes reassociation, and additionary ADP after the cation treatment further increases the binding of monoclonal antibodies. 4. Prolonged incubation of intact platelets with EDTA at 37 degrees C induces a peculiar change of structure in the GPIIb/IIIa.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0049-3848
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-58
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Effects of EDTA on the membrane glycoproteins IIb-IIIa complex--analysis using flow cytometry.
pubmed:affiliation
First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
pubmed:publicationType
Journal Article