Linked Life Data

3116156

Source:http://linkedlifedata.com/resource/pubmed/id/3116156

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1987-11-5
pubmed:abstractText
Previous work has shown that the side wall of a Gram-positive rod is initially laid down as a compact layer inside the older wall. It is then stretched as it comes to bear tension due to the osmotic pressure inside the cell. If the polar wall is likewise capable of a degree of expansion, then no new murein need be added while the planar cross-wall splits and converts into two poles. In Bacillus subtilis mutant strain FJ6, which is deficient in autolytic enzymes, pole formation can be caused by addition of exogenous muramidase (10 micrograms hen egg white lysozyme ml-1 for 10 min at 35 degrees C). This strain grows as long filaments with many completed cross-walls, but enzymic treatment caused the formation of many new poles of normal morphology as judged by thin section electron microscopy. Fully separated poles of normal appearance were also found when more than 100 times the MIC (1 microgram ml-1) of vancomycin was added to block wall growth totally and rapidly 10 min before the addition of lysozyme. We conclude, therefore, that no new murein is needed in the conversion of the flat septum into poles and that the unstressed cross-wall is capable of the necessary expansion.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1287
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3441-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Normal pole formation during total inhibition of wall synthesis of Bacillus subtilis.
pubmed:affiliation
Department of Biology, Indiana University, Bloomington 47405.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't