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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1987-11-20
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pubmed:abstractText |
We investigated the influence of various fixatives on monoclonal anti-BrdUrd antibody binding of BrdUrd-substituted DNA in tissue sections of routinely processed mouse small intestine after in vivo administration of BrdUrd. For denaturing fixatives such as ethanol or Carnoy's fluid, a standard denaturation protocol showed specific crypt cell labeling. With cross-linking agents such as formalin and glutaraldehyde, a remarkable increase in staining intensity was obtained after tissue digestion with pepsin before acid denaturation. The optimal pepsin concentration was determined for maximal immunoreactivity combined with acceptable morphology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1554
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1343-5
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:3116075-Animals,
pubmed-meshheading:3116075-Antibodies, Monoclonal,
pubmed-meshheading:3116075-Bromodeoxyuridine,
pubmed-meshheading:3116075-Cross-Linking Reagents,
pubmed-meshheading:3116075-DNA,
pubmed-meshheading:3116075-Fixatives,
pubmed-meshheading:3116075-Formaldehyde,
pubmed-meshheading:3116075-Frozen Sections,
pubmed-meshheading:3116075-Glutaral,
pubmed-meshheading:3116075-Immunoenzyme Techniques,
pubmed-meshheading:3116075-Intestine, Small,
pubmed-meshheading:3116075-Mice,
pubmed-meshheading:3116075-Mice, Inbred BALB C,
pubmed-meshheading:3116075-Pepsin A,
pubmed-meshheading:3116075-Protein Denaturation
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pubmed:year |
1987
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pubmed:articleTitle |
Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry.
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pubmed:affiliation |
Department of Internal Medicine, University of Limburg, Maastricht, The Netherlands.
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pubmed:publicationType |
Journal Article
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