Linked Life Data

3115589

Source:http://linkedlifedata.com/resource/pubmed/id/3115589

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-11-13
pubmed:abstractText
The presence of chondroitin sulfate in secretory granules of the rat parotid gland and its saliva was revealed by radioactive sulfate incorporation, followed by isolation and partial characterization of the sulfated species contained within the granules and in the parotid saliva. 35SO4 was incorporated into chromatographically identical macromolecular material both in vitro, in a gland-slice system followed by isolation of granule contents, and in vivo as measured in the pure parotid secretion following intravenous administration of 35SO4=. The majority of the 35SO4= label appeared in a peak in the region where the family of acidic proline-rich proteins elute from a DEAE-Sephadex A-50 column. Papain digestion freed the sulfated material from the bulk of protein present in this peak, leaving sulfate-labelled material that chromatographed on Sepharose CL6B as a single peak corresponding to a molecular weight of 13,000 daltons. The ratio of uronic acid to amino sugar in this sulfated peak was 0.56. The sulfated material was susceptible to degradation by chondroitinase AC. The presence of this chondroitin sulfate in secretory granules and saliva is consistent with previous suggestions that sulfated polyanions may play a role in formation and maturation of secretory granules.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
250
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
221-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
The presence of chondroitin sulfate in parotid secretory granules and saliva of the rat.
pubmed:affiliation
Department of Oral Biology, University of Washington, Seattle 98195.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.