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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
1987-10-22
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pubmed:abstractText |
A quantitative solid phase immunoassay has been developed for the determination of the mass of electrophoretically separated prebeta apolipoprotein A-I (apoA-I) in human plasma. Conditions have been identified for the quantitative transfer and immunoblotting of the apolipoprotein in the absence of organic solvents or detergents. In normolipidemic plasma, the prebeta-migrating fraction of apoA-I represented 4.2 +/- 1.8% of total apoA-I (61 +/- 26 micrograms of apoA-I per ml of plasma). Significantly higher levels were found in hypercholesterolemia of genetic origin, in primary and secondary hypertriglyceridemia, and in congenital lecithin:cholesterol acyltransferase deficiency. In all cases prebeta-migrating apoA-I consisted in large part of low molecular weight lipoprotein species, compared to the size of the major, alpha-migrating apoA-I fraction.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoprotein A-I,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins A,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-2275
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
778-86
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3114402-Apolipoprotein A-I,
pubmed-meshheading:3114402-Apolipoproteins A,
pubmed-meshheading:3114402-Blood Protein Electrophoresis,
pubmed-meshheading:3114402-Humans,
pubmed-meshheading:3114402-Hyperlipidemias,
pubmed-meshheading:3114402-Immunoelectrophoresis, Two-Dimensional,
pubmed-meshheading:3114402-Immunoglobulin G,
pubmed-meshheading:3114402-Iodine Radioisotopes,
pubmed-meshheading:3114402-Lipoproteins, HDL,
pubmed-meshheading:3114402-Molecular Weight,
pubmed-meshheading:3114402-Radioimmunoassay
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pubmed:year |
1987
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pubmed:articleTitle |
Prebeta-migrating high density lipoprotein: quantitation in normal and hyperlipidemic plasma by solid phase radioimmunoassay following electrophoretic transfer.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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