|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1987-7-17
|
|
pubmed:abstractText |
Virulent toxigenic and avirulent non-toxigenic strains of Clostridium difficile gave a positive result in the latex agglutination test (LAT) for C difficile toxin A (D-1). Similar concentrations of latex agglutinating antigen were produced by these strains in vivo. Positive reactions were also given by C sporogenes, proteolytic C botulinum Types A, B, and A/F, and Bacteroides assaccharolyticus. The latex agglutinating antigen was denatured by boiling for 10 minutes, but not by heating at 56 degrees C for 30 minutes. The reaction was abolished by incubation of test material with crude C difficile antitoxin but not with other clostridial antitoxins or specific antitoxin to C difficile toxin A. The latex agglutinating antigen present in C difficile eluted between 0.39% and 0.47% M sodium chloride, and that produced by the other clostridia, between 0.35% and 0.43% M sodium chloride by fast protein liquid chromatography. The latex agglutinating antigen of C difficile was neither cytotoxic nor mouse lethal and was distinct from toxin A and toxin B. In the analysis of faecal specimens from patients with diarrhoea the latex agglutination test correlated better with the presence of C difficile than with toxin B and detected both toxigenic and non-toxigenic strains. The latex agglutination test should only be used in the laboratory as an alternative to culture for C difficile and not as a method for the detection of C difficile toxins.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-3086678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-3093537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-4009689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-6101243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-6490824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-6500693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-6508777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-6764763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-6829506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-7068210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-7251895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3108333-7441010
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0021-9746
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
40
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
573-80
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3108333-Animals,
pubmed-meshheading:3108333-Bacterial Proteins,
pubmed-meshheading:3108333-Bacterial Toxins,
pubmed-meshheading:3108333-Chromatography, Ion Exchange,
pubmed-meshheading:3108333-Clostridium,
pubmed-meshheading:3108333-Cricetinae,
pubmed-meshheading:3108333-Cytotoxins,
pubmed-meshheading:3108333-Diarrhea,
pubmed-meshheading:3108333-Enterotoxins,
pubmed-meshheading:3108333-Feces,
pubmed-meshheading:3108333-Hot Temperature,
pubmed-meshheading:3108333-Humans,
pubmed-meshheading:3108333-Latex Fixation Tests,
pubmed-meshheading:3108333-Mice,
pubmed-meshheading:3108333-Virulence
|
|
pubmed:year |
1987
|
|
pubmed:articleTitle |
Analysis of latex agglutination test for Clostridium difficile toxin A (D-1) and differentiation between C difficile toxins A and B and latex reactive protein.
|
|
pubmed:publicationType |
Journal Article
|
