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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1987-6-26
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pubmed:abstractText |
GTP-binding proteins have been proposed to be involved in some secretory processes. Bordetella pertussis toxin is known to catalyze ADP-ribosylation of several GTP-binding proteins. In this paper, the subcellular localization of B. pertussis toxin substrates has been explored in chromaffin cells of bovine adrenal medulla. With appropriate gel electrophoresis conditions, three ADP-ribosylated substrates of 39, 40 and 41 kDa were detectable in both plasma and granule membranes. The more intense labelling occurred on the 40 kDa component, while the 41 kDa species exhibited electrophoretic mobility similar to that of Gi alpha. Significant immunoreactivity with anti-Go alpha antibodies was detected at the level of the 39 kDa faster component. The association of G-proteins with granule and plasma membranes suggests the involvement of these proteins in the exocytotic process or in its regulation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
215
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
339-44
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:3108031-Adenosine Diphosphate Ribose,
pubmed-meshheading:3108031-Adrenal Medulla,
pubmed-meshheading:3108031-Animals,
pubmed-meshheading:3108031-Autoradiography,
pubmed-meshheading:3108031-Cattle,
pubmed-meshheading:3108031-Cell Membrane,
pubmed-meshheading:3108031-Centrifugation, Density Gradient,
pubmed-meshheading:3108031-Chromaffin Granules,
pubmed-meshheading:3108031-Chromaffin System,
pubmed-meshheading:3108031-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3108031-GTP-Binding Proteins,
pubmed-meshheading:3108031-Immunochemistry,
pubmed-meshheading:3108031-Pertussis Toxin,
pubmed-meshheading:3108031-Virulence Factors, Bordetella
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pubmed:year |
1987
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pubmed:articleTitle |
Presence of three pertussis toxin substrates and Go alpha immunoreactivity in both plasma and granule membranes of chromaffin cells.
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pubmed:publicationType |
Journal Article
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