Linked Life Data

3104396

Source:http://linkedlifedata.com/resource/pubmed/id/3104396

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1987-4-27
pubmed:abstractText
The effect of various concentrations of oestradiol and progesterone on DNA synthesis in endometrial endothelial cells derived from human decidua has been studied in vitro by means of a microfluorometric technique and [3H]thymidine incorporation. Oestradiol in concentrations of 10-40 ng/ml was found to significantly stimulate DNA synthesis in the cells whereas progesterone significantly decreased the incorporation of [3H]thymidine. A highly significant correlation (P less than 0.01) was found in the oestrogen-treated samples between the [3H]thymidine incorporation and the ethidium bromide fluorescence after the removal of RNA. No such correlation was found in the progesterone-treated samples. A microfluorometric assessment of the anti-haemophilic antigen Factor VIII following immunofluorescence labelling suggested an increase in the synthesis of this factor after oestradiol treatment whereas progesterone administration did not show any effect. Although no direct extrapolation can be made from the results of the in vitro studies to in vivo conditions the endothelial cell culture system provides a useful model for testing the effect of mitotic mediators and compounds interfering with intracellular protein synthesis. It may therefore contribute to a better understanding of the bleeding mechanism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
207-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Effects of oestradiol and progesterone on the synthesis of DNA and the anti-haemophilic factor VIII antigen in human endometrial endothelial cells in vitro: a pilot study.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't