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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1987-4-22
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pubmed:abstractText |
Cross-linking of receptor bound IgE antibodies by multivalent antigen (DNP8-BSA) on PB-3c cells leads to an increase of cytosolic calcium ((Ca2+)i). Active tumor promoting phorbol esters and teleocidin which specifically activate the phospholipid Ca2+-sensitive protein kinase (PKC), inhibited the antigen-mediated rise in (Ca2+)i and induced a time and dose-dependent translocation of cytosolic PKC to membranes of the PB-3c cells as determined by enzyme activity or immunoblotting using a polyclonal anti-PKC antibody. This TPA concentration did not affect the subcellular distribution of PKC, although 1 nM of 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibited to 50% the antigen-mediated increase in (Ca2+)i. The concentration of TPA required to induce a half-maximal subcellular redistribution of immunodetectable PKC activity was an order of magnitude greater than the half-maximal dose required to inhibit the antigen-mediated increase in (Ca2+)i. These data demonstrate that the TPA-dependent activation of PKC is not directly coupled to its translocation to membranes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Lyngbya Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/teleocidin
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
252-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:3103618-Antigen-Antibody Complex,
pubmed-meshheading:3103618-Antigens,
pubmed-meshheading:3103618-Biological Transport,
pubmed-meshheading:3103618-Calcium,
pubmed-meshheading:3103618-Cell Line,
pubmed-meshheading:3103618-Cell Membrane,
pubmed-meshheading:3103618-Cytosol,
pubmed-meshheading:3103618-Immunoglobulin E,
pubmed-meshheading:3103618-Kinetics,
pubmed-meshheading:3103618-Lyngbya Toxins,
pubmed-meshheading:3103618-Mast Cells,
pubmed-meshheading:3103618-Phorbol Esters,
pubmed-meshheading:3103618-Protein Kinase C
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pubmed:year |
1987
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pubmed:articleTitle |
Translocation of protein kinase C is not required to inhibit the antigen-induced increase of cytosolic calcium in a mast cell line.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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