|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1987-4-1
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The germ-line elements for the variable (V) and joining (J) regions of the kappa chains (V kappa-Ars, J kappa 1) that give rise to the productive allele for the A/J mouse light chains of Ars-A monoclonal antibodies (directed against the hapten p-azophenylarsonate) have been cloned and sequenced as well as a rearranged Ars-A light chain gene. Using the V kappa-Ars gene as a hybridization probe, we provide evidence that the V kappa 10 family is relatively small and that only one member gives rise to the productive allele in all Ars-A antibodies. An unusual feature of Ars-A light chains is that all contain an arginine at position 96, the V-J junctional position. We had shown by chain recombination that arginine-96 was essential for antigen binding. In the present study, an arginine codon was not found either at the 3' end of the V kappa-Ars gene segment or at the 5' end of J kappa 1. However, an arginine codon (CGG) can easily be generated by recombination between these two germ-line elements. Thus, we document that junctional diversity through intracodonic recombination can be crucial to the antibody binding function of the resulting molecule.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-111144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-3011910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-3081433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-3085101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-3927173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6166399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6222379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6403356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6416834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6431763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6453743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6546718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6712161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6747289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6791833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6801658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6801765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6806358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6877353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-6985352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3103124-7231520
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
84
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1085-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3103124-Alleles,
pubmed-meshheading:3103124-Animals,
pubmed-meshheading:3103124-Antibodies, Monoclonal,
pubmed-meshheading:3103124-Arginine,
pubmed-meshheading:3103124-Cloning, Molecular,
pubmed-meshheading:3103124-DNA,
pubmed-meshheading:3103124-Genes,
pubmed-meshheading:3103124-Hybridomas,
pubmed-meshheading:3103124-Immunoglobulin Variable Region,
pubmed-meshheading:3103124-Immunoglobulin kappa-Chains,
pubmed-meshheading:3103124-Mice,
pubmed-meshheading:3103124-Mice, Inbred Strains,
pubmed-meshheading:3103124-Nucleic Acid Hybridization,
pubmed-meshheading:3103124-Recombination, Genetic
|
|
pubmed:year |
1987
|
|
pubmed:articleTitle |
V kappa and J kappa gene segments of A/J Ars-A antibodies: somatic recombination generates the essential arginine at the junction of the variable and joining regions.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
