Linked Life Data

3098354

Source:http://linkedlifedata.com/resource/pubmed/id/3098354

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1987-1-27
pubmed:abstractText
In vitro tissue culture experiments are routinely performed at atmospheric oxygen tension (21%), although most cells experience lower oxygen tensions in vivo. The optimal oxygen tension for the survival of neurons and synthesis of neurofilament (NF) was determined using dissociated neonatal rat cerebral cortex and monoclonal antibodies to the 68 kDa and 200 kDa subunits of NF. Maximum production of each subunit determined by Enzyme-Linked Immunosorbant Assay occurred at 9% O2. Neuronal survival determined by immunohistochemistry was also maximal at 9% O2. CNS neurons are highly sensitive to oxygen tension and grow best at levels approximating capillary rather than atmospheric pO2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
384
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-203
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Enhanced survival of rat neonatal cerebral cortical neurons at subatmospheric oxygen tensions in vitro.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't