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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1987-1-14
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA clone to human 4-beta-galactosyltransferase (EC 2.4.1.38) was isolated from a human liver lambda gt11 expression library by using a monospecific polyclonal antiserum to affinity-purified bovine enzyme. The authenticity of this cDNA clone has been demonstrated by several criteria. Under conditions of chronic treatment with the beta-adrenergic receptor agonist isoproterenol, rat parotid glands show an approximately 10-fold increase in 4-beta-galactosyltransferase activity. The increased enzyme activity was reflected in dot-blot analysis of control and isoproterenol-treated rat parotid RNA by using the human cDNA as probe. Hybrid-selection and in vitro translation identified a protein product with a molecular mass of 47 kDa that was immunoprecipitated with the bovine antiserum. The full-length human cDNA clone was then isolated and the DNA sequence for the NH2-terminal portion of the protein was deduced. Comparison of the NH2-terminal protein sequence from the bovine protein with that of the human cDNA clone confirmed its identity. In addition, the human cDNA clone was used to localize the gene for 4-beta-galactosyltransferase to human chromosome 4 by Southern analysis of a somatic cell hybrid panel.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-191828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-2419911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-2986678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-3014508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-396438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-4074366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-4326780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-4547468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-4698230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-476621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-5165268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-5238979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-5440844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-5476326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-598253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6089358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6093508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6159478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6201486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6316146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6356359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6572945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6691137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-6806281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-7005145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-7096324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-822353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3097639-92031
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8918-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3097639-Amino Acid Sequence,
pubmed-meshheading:3097639-Base Sequence,
pubmed-meshheading:3097639-Chromosome Mapping,
pubmed-meshheading:3097639-Chromosomes, Human, Pair 4,
pubmed-meshheading:3097639-Cloning, Molecular,
pubmed-meshheading:3097639-DNA,
pubmed-meshheading:3097639-Galactosyltransferases,
pubmed-meshheading:3097639-Gene Expression Regulation,
pubmed-meshheading:3097639-Humans,
pubmed-meshheading:3097639-Isoproterenol,
pubmed-meshheading:3097639-Parotid Gland,
pubmed-meshheading:3097639-RNA, Messenger
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pubmed:year |
1986
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pubmed:articleTitle |
Molecular cloning and chromosomal localization of human 4-beta-galactosyltransferase.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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