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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1986-12-19
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pubmed:databankReference |
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pubmed:abstractText |
The Abelson murine leukemia virus (A-MuLV) transformed cell line 300-19 was derived from the bone marrow of an adult NIH/Swiss outbred mouse. The original 300-19 clonal isolate carried DHH rearrangements of both JH alleles, a molecular genotype characteristic of early pre-B cells. During propagation in culture, the 300-19 line frequently generates secondary rearrangements of its JH alleles including rearrangements which append VH segments to the pre-existing DJH complexes to form complete VHDJH variable region genes and secondary D to JH rearrangements which replace the pre-existing DJH rearrangement by joining an upstream D to a downstream JH. The two types of secondary rearrangement events occur at approximately equal frequency. Approximately 30% of the VH to DJH joins lead to the production of mu heavy chains providing support for a regulated model of allelic exclusion. Like pre-B cell lines from other origins, the 300-19 line preferentially utilized VH gene segments from the more JH-proximal (3') families to form VHDJH rearrangements. However, the VH segments preferentially employed by 300-19 were from a different family than those previously demonstrated to be utilized by pre-B lines of BALB/c origin; we relate these different utilization patterns to differences in the organization of the more 3' VH families between the two strains. The initial DJH rearrangements of the 300-19 line employed more 3' (JH-proximal) D segments; however, the DJH replacements preferentially employed the most 5' D segment. We discuss this phenomenon in the context of a mechanism which may target recombinase to regions of the chromosome more 5' to the D locus (VH-containing regions) once an initial DJH complex is formed.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-2991895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-2993908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-2998759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-3081433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-3085692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-3484682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-3930970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-3975629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6086308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6086934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6092095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6092962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6095102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6277505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6287467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6291785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6316256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6407007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6417229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6422305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6774258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6783962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6798155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3096716-6975306
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2131-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3096716-Abelson murine leukemia virus,
pubmed-meshheading:3096716-Alleles,
pubmed-meshheading:3096716-Amino Acid Sequence,
pubmed-meshheading:3096716-Animals,
pubmed-meshheading:3096716-B-Lymphocytes,
pubmed-meshheading:3096716-Base Sequence,
pubmed-meshheading:3096716-Bone Marrow Cells,
pubmed-meshheading:3096716-Cell Differentiation,
pubmed-meshheading:3096716-Cell Transformation, Neoplastic,
pubmed-meshheading:3096716-Cells, Cultured,
pubmed-meshheading:3096716-Genes,
pubmed-meshheading:3096716-Immunoglobulin Heavy Chains,
pubmed-meshheading:3096716-Immunoglobulin Variable Region,
pubmed-meshheading:3096716-Mice,
pubmed-meshheading:3096716-Mice, Inbred Strains
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pubmed:year |
1986
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pubmed:articleTitle |
VHDJH formation and DJH replacement during pre-B differentiation: non-random usage of gene segments.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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