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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1986-12-5
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pubmed:abstractText |
The structural gene for diphtheria toxin, tox, has been modified at its Sph I site by the introduction of an oligonucleotide linker encoding a unique Pst I restriction endonuclease site and a synthetic oligonucleotide encoding alpha-melanocyte-stimulating hormone (alpha-MSH). The resulting fusion gene directs the expression of a diphtheria toxin-related alpha-MSH hybrid protein in which the diphtheria toxin receptor-binding domain has been replaced with alpha-MSH sequences. The chimeric toxin has been partially purified from periplasmic extracts of recombinant Escherichia coli K-12 and has been found to be selectively toxic for alpha-MSH receptor-positive human malignant melanoma NEL-M1 cells in vitro.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-14192657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-174112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-190237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-212233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-281682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-286111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-3002340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-3886165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-3919942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-4857870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-4896022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-4999827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-5001205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-508328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-5271752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-5856535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6167984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6183210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6218613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6256086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6266278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6270058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6270061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6295879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6296105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6300771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6313613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6314249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6316330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6317684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6348945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6403984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6766935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-6776122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-7085634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-7251138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3095831-762110
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8258-62
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3095831-ADP Ribose Transferases,
pubmed-meshheading:3095831-Ammonium Chloride,
pubmed-meshheading:3095831-Cell Line,
pubmed-meshheading:3095831-Diphtheria Toxin,
pubmed-meshheading:3095831-Genes,
pubmed-meshheading:3095831-Humans,
pubmed-meshheading:3095831-Melanocyte-Stimulating Hormones,
pubmed-meshheading:3095831-Melanoma,
pubmed-meshheading:3095831-Molecular Weight,
pubmed-meshheading:3095831-Pentosyltransferases,
pubmed-meshheading:3095831-Recombinant Fusion Proteins,
pubmed-meshheading:3095831-Recombinant Proteins
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pubmed:year |
1986
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pubmed:articleTitle |
Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related alpha-melanocyte-stimulating hormone fusion protein.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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