Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1986-9-25
|
pubmed:abstractText |
Collagen (soluble bovine tendon type I) coated onto microtiter plates binds von Willebrand antigen (vW:Ag) in a dose-dependent manner. An ELISA test was set up with both antibody and collagen coated microtiter plates. Test specimens assayed were: 1) normal plasmas, 2) type I vW plasmas, 3) type IIa vW plasmas, and 4) factor VIII concentrates (KoateR, Cutter; Conco-VIII, Green Cross). Normal and type I vW plasmas exhibited comparable values for vW:Ag in binding studies to both collagen and antibody-coated plates. Type IIa vW plasmas demonstrated decreased (less than 1/2) collagen to antibody-binding ratios. Ristocetin cofactor (VIII:RCO) levels in type IIa vW plasmas correlated with quantified collagen-binding levels. Factor VIII concentrates show variable results when comparing collagen and antibody-binding levels. A comparison of vW:Ag ELISA (antibody) with VIII:RCO shows ratios of 2:1 (KoatR) or 20:1 (Conco-VIII). Collagen-binding ELISA levels in concentrates show parallel decreases, reflecting presumed binding to collagen of only the high M.W. multimers. The vW:Ag collagen binding ELISA represents a possible replacement assay for the laborious and imprecise VIII:RCO method of measurement of in vitro vWf functional activity.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Factor VIII,
http://linkedlifedata.com/resource/pubmed/chemical/Von Willebrand antigen,
http://linkedlifedata.com/resource/pubmed/chemical/von Willebrand Factor
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0049-3848
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
43
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
303-11
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:3090735-Antibodies,
pubmed-meshheading:3090735-Antigens,
pubmed-meshheading:3090735-Collagen,
pubmed-meshheading:3090735-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3090735-Factor VIII,
pubmed-meshheading:3090735-Female,
pubmed-meshheading:3090735-Humans,
pubmed-meshheading:3090735-Male,
pubmed-meshheading:3090735-von Willebrand Diseases,
pubmed-meshheading:3090735-von Willebrand Factor
|
pubmed:year |
1986
|
pubmed:articleTitle |
An ELISA test for the binding of von Willebrand antigen to collagen.
|
pubmed:publicationType |
Journal Article,
In Vitro
|