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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1986-9-17
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pubmed:databankReference |
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pubmed:abstractText |
cDNA clones of the mRNA coding for tissue-type plasminogen activator (t-PA) have been obtained and their nucleotide sequences compared to those reported previously. A gene coding for t-PA has been reconstructed and inserted into vectors for expression in prokaryotic cells. Relatively high levels of t-PA accumulated in inclusion bodies in Escherichia coli containing an optimized expression plasmid, but only a small proportion of the insoluble protein was recovered as active enzyme using a variety of solubilization procedures.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0735-1313
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
279-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3090401-Amino Acid Sequence,
pubmed-meshheading:3090401-Base Sequence,
pubmed-meshheading:3090401-Cell Line,
pubmed-meshheading:3090401-Cloning, Molecular,
pubmed-meshheading:3090401-DNA,
pubmed-meshheading:3090401-DNA, Recombinant,
pubmed-meshheading:3090401-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3090401-Enzyme Activation,
pubmed-meshheading:3090401-Escherichia coli,
pubmed-meshheading:3090401-Gene Expression Regulation,
pubmed-meshheading:3090401-Humans,
pubmed-meshheading:3090401-Plasmids,
pubmed-meshheading:3090401-Tissue Plasminogen Activator
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pubmed:year |
1986
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pubmed:articleTitle |
Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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