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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1986-9-17
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pubmed:abstractText |
Human uroepithelial (T24) cells were incubated for 24 h in the presence of various concentrations of human recombinant gamma interferon (Hu-rIFN-gamma) and then infected with the 6BC strain of Chlamydia psittaci. This resulted in a reduction of intracellular chlamydial inclusion development in proportion to the concentration of Hu-rIFN-gamma present when Giemsa-stained cells were examined by light microscopy 24 h after infection. When tryptophan was added to Hu-rIFN-gamma-treated cells just after infection, reversal of the Hu-rIFN-gamma-mediated inhibition occurred in proportion to the concentration of tryptophan added. Addition of either isoleucine or lysine did not result in reversal of the antichlamydial state. Transport of L-[3H]tryptophan into acid-soluble intracellular pools was found to be greatly enhanced in Hu-rIFN-gamma-treated T24 cells compared with the rates measured for untreated cells. Transport of [3H]leucine was not increased in treated cells. Cells treated with Hu-rIFN-gamma also degraded L-[3H]tryptophan to catabolites that cochromatographed with N-formylkynurenine and kynurenine as measured by high-performance liquid chromatography. We conclude that Hu-rIFN-gamma-mediated inhibition of intracellular C. psittaci replication in T24 cells occurs by depletion of the essential amino acid tryptophan, most likely via the induction of indoleamine-2,3-dioxygenase, the initial enzyme of tryptophan catabolism.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-1095493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-2416307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-2985506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-3928493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-3934279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-541438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6165986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6256463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6313807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6411853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6412144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6417024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6417262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6421982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6422465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6425215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6432701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6601625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6840848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-6864162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-7054284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-7299350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3089936-7400757
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
347-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1986
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pubmed:articleTitle |
Induction of tryptophan catabolism is the mechanism for gamma-interferon-mediated inhibition of intracellular Chlamydia psittaci replication in T24 cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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