|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1986-7-25
|
|
pubmed:abstractText |
We report the use of UDPgalactose:N-acetyl-D-glucosaminyl-glycopeptide 4-beta-D-galactosyl-transferase (EC 2.4.1.38), purified from bovine milk, to detect nonreducing terminal N-acetylglucosamine residues on glycoproteins immobilized on nitrocellulose by electrophoretic transfer from sodium dodecyl sulfate-polyacrylamide gels. Soluble galactosyltransferase incorporates radiolabeled galactose from the substrate UDP-[6-3H]galactose into the appropriate immobilized acceptor with high specificity. Incorporation is proportional to substrate amount and is saturable with time. The kinetics of labeling are independent of substrate amount. Half-maximal incorporation occurs by 4 h and saturation occurs by 16 h. We have used galactosyltransferase as a probe (i) to verify the presence of nonreducing terminal N-acetylglucosamine residues in bovine rod outer segment membrane rhodopsin and in several glycoproteins in F9 murine teratocarcinoma cells and (ii) to detect previously reported endo-beta-N-acetylglucosaminidase activity in a commercial preparation of endoglycosidase F.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0003-2697
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
154
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
460-9
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:3089057-Acetylglucosamine,
pubmed-meshheading:3089057-Acetylglucosaminidase,
pubmed-meshheading:3089057-Animals,
pubmed-meshheading:3089057-Cattle,
pubmed-meshheading:3089057-Cell Line,
pubmed-meshheading:3089057-Collodion,
pubmed-meshheading:3089057-Galactosyltransferases,
pubmed-meshheading:3089057-Glucosamine,
pubmed-meshheading:3089057-Glycoproteins,
pubmed-meshheading:3089057-Glycoside Hydrolases,
pubmed-meshheading:3089057-Kinetics,
pubmed-meshheading:3089057-Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase,
pubmed-meshheading:3089057-Mice,
pubmed-meshheading:3089057-Milk,
pubmed-meshheading:3089057-Neoplasm Proteins,
pubmed-meshheading:3089057-Oxidation-Reduction,
pubmed-meshheading:3089057-Rhodopsin,
pubmed-meshheading:3089057-Solubility,
pubmed-meshheading:3089057-Teratoma
|
|
pubmed:year |
1986
|
|
pubmed:articleTitle |
The use of galactosyltransferase to probe nitrocellulose-immobilized glycoproteins for nonreducing terminal N-acetylglucosamine residues.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
