Linked Life Data

3084547

Source:http://linkedlifedata.com/resource/pubmed/id/3084547

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-5-30
pubmed:abstractText
Accurate evaluation of bacterial colonization as a predictive index for wound sepsis has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of biopsy tissue homogenate. The method, while useful to the physician, is both labor intensive and expensive. In this study 78 eschar biopsies were cultured by a semiquantitative technique that involved the use of 0.1- and 0.01-ml samples of inocula and by the serial dilution method. Exact colony counts from the semiquantitative culture method were available only from cultures containing 10(4) to 10(6) CFU/g of tissue. Other colony counts were reported as less than 10(4) or greater than 10(6) CFU/g. Agreement by category of colony counts between the two methods was 96%. For prediction of wound sepsis, the semiquantitative procedure had a positive predictive value of 100% and a negative predictive value of 93.7%. This method also resulted in an approximately 30% reduction of work units (as defined by the College of American Pathologists) and a 60% reduction in the amount of media for specimen processing. Therefore, this semiquantitative culture technique provides accurate information to the physician while saving both time and materials.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-1255833, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-14160479, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-326080, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-378056, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-386811, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-4588085, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-4898100, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-5413463, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-6337522, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-7013468, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-7038133, http://linkedlifedata.com/resource/pubmed/commentcorrection/3084547-7125790
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
258-61
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Comparison of quantitative and semiquantitative culture techniques for burn biopsy.
pubmed:publicationType
Journal Article, Comparative Study